PMID: 9169603May 1, 1997Paper

Biochemical characterization of the arginine-specific proteases of Porphyromonas gingivalis W50 suggests a common precursor

The Biochemical Journal
M RangarajanM A Curtis

Abstract

Extracellular proteases of Porphyromonas gingivalis specific for arginyl peptide bonds are considered to be important virulence factors in periodontal disease. In order to determine the number, inter-relationship and kinetic properties of these proteases, extracellular enzymes with this peptide-bond specificity were purified and characterized from P. gingivalis W50. Three forms, which we denote RI, RI-A and RI-B, accounted for all of the activity in the supernatant. All three enzymes contain an alpha chain of approximately 54 kDa with the same N-terminal amino acid sequence. RI is a heterodimer of non-covalently linked alpha and beta chains which migrate to the same position on SDS/PAGE but which can be resolved by 8 M urea/PAGE. RI-A and RI-B are both monomeric, but the molecular mass of RI-B (70-80 kDa) is significantly increased due to post-translational modification with lipopolysaccharide. All forms show absolute specificity for peptide bonds with Arg in the P1 position and are also capable of hydrolysing N-terminal Arg and C-terminal Arg-Arg peptide bonds. Thus they show limited amino- and carboxy-peptidase activity. For the hydrolysis of Nalpha-benzoyl-L-Arg-p-nitroanilide, the pH optimum is 8.0 at 30 degrees C. The Vmax...Continue Reading

Citations

Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A CurtisM Rangarajan
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
Dec 19, 2009·Periodontology 2000·Rishi D PathiranaEric C Reynolds
Jun 14, 2013·PloS One·N Laila HuqEric C Reynolds
Oct 27, 2015·Clinical and Experimental Dental Research·Kalid N HosnEdwin L Thomas
Sep 14, 2002·Infection and Immunity·Margaret A ScraggMichael A Curtis
Jan 21, 2017·Molecular Oral Microbiology·M RangarajanM A Curtis
Mar 4, 2000·Journal of Periodontal Research·M A CurtisJ Aduse-Opoku
Sep 24, 2013·Journal of Bacteriology·Minnie RangarajanMichael A Curtis
Jun 13, 2017·Frontiers in Cellular and Infection Microbiology·Anna M LasicaJan Potempa
May 18, 2012·The Journal of Biological Chemistry·Michelle D GlewEric C Reynolds
May 7, 2009·Future Microbiology·Rebecca E FitzpatrickRobert N Pike
Oct 16, 1999·Periodontology 2000·S C HoltC A Genco
Sep 3, 2021·Journal of Chemical Information and Modeling·Santiago MovillaVicent Moliner
Dec 24, 2008·Biochemical and Biophysical Research Communications·Tomonori SuzukiTohru Ohyama
May 1, 2010·Biochimie·Neil D Rawlings

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