Biochemical characterization of the major sorghum grain peroxidase

The FEBS Journal
Mamoudou H DickoWillem J H van Berkel

Abstract

The major cationic peroxidase in sorghum grain (SPC4) , which is ubiquitously present in all sorghum varieties was purified to apparent homogeneity, and found to be a highly basic protein (pI approximately 11). MS analysis showed that SPC4 consists of two glycoforms with molecular masses of 34,227 and 35,629 Da and it contains a type-b heme. Chemical deglycosylation allowed to estimate sugar contents of 3.0% and 6.7% (w/w) in glycoform I and II, respectively, and a mass of the apoprotein of 33,246 Da. High performance anion exchange chromatography allowed to determine the carbohydrate constituents of the polysaccharide chains. The N-terminal sequence of SPC4 is not blocked by pyroglutamate. MS analysis showed that six peptides, including the N-terminal sequence of SPC4 matched with the predicted tryptic peptides of gene indice TC102191 of sorghum chromosome 1, indicating that TC102191 codes for the N-terminal part of the sequence of SPC4, including a signal peptide of 31 amino acids. The N-terminal fragment of SPC4 (213 amino acids) has a high sequence identity with barley BP1 (85%), rice Prx23 (90%), wheat WSP1 (82%) and maize peroxidase (58%), indicative for a common ancestor. SPC4 is activated by calcium ions. Ca2+ binding i...Continue Reading

References

Apr 1, 1992·Plant Molecular Biology·A JohanssonK G Welinder
Nov 1, 1991·Biochemical Society Transactions·B N Green, R W Oliver
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D BuffardR Esnault
Nov 15, 1981·Analytical Biochemistry·A S EdgeP Weber
Jan 1, 1981·Journal of Agricultural and Food Chemistry·C W Glennie
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Sep 1, 1996·Archives of Biochemistry and Biophysics·D A Converso, M E Fernández
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Aug 1, 1997·European Journal of Biochemistry·T TeichmannK Palme
Sep 26, 1997·Molecular Plant-microbe Interactions : MPMI·J M ChittoorF F White
Nov 25, 1998·Protein Science : a Publication of the Protein Society·C P van MierloE Steensma
May 23, 2001·Journal of Agricultural and Food Chemistry·G OudgenoegC Laane
Sep 21, 2001·Applied Biochemistry and Biotechnology·M H DickoG Beldman
May 25, 2002·European Journal of Biochemistry·Anabel RodríguezValery L Shnyrov
Jun 13, 2002·Journal of Agricultural and Food Chemistry·Mamoudou H DickoAlphons G J Voragen
Jul 19, 2002·Analytical Biochemistry·Mamoudou H DickoAlfons G J Voragen
Sep 25, 2003·Annual Review of Plant Biology·Wout BoerjanMarie Baucher
Nov 26, 2003·Current Opinion in Genetics & Development·Andrew H PatersonBrad A Chapman
Jan 8, 2004·Journal of Molecular Evolution·Laurent Duroux, Karen G Welinder
Jun 9, 2004·Phytochemistry·Joseph M Awika, Lloyd W Rooney
Jul 29, 2004·Phytochemistry·Filippo PassardiChristophe Dunand
Oct 14, 2004·Journal of Agricultural and Food Chemistry·Carmen G BoeriuAlphons G J Voragen

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Citations

Feb 25, 2011·Plant Physiology and Biochemistry : PPB·Angela CardinaliGabriella Sonnante
Dec 17, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Chukwudi I NnamchiEmma L Raven
Jul 14, 2011·Proteomics·Mohsen MohammadiLinda J Harris

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