Biochemical coupling between the DrrA and DrrB proteins of the doxorubicin efflux pump of Streptomyces peucetius.

The Journal of Biological Chemistry
Parjit Kaur, J Russell

Abstract

The drrAB operon of Streptomyces peucetius encodes for resistance to the antibiotics doxorubicin and daunorubicin. Subcloning of the drrAB genes in Escherichia coli has previously been shown to result in expression of DrrA and DrrB proteins and resistance to doxorubicin in a sensitive strain of E. coli. DrrA, a peripheral membrane protein, binds ATP in a UV-catalyzed reaction in a doxorubicin-dependent manner; DrrB, a hydrophobic protein, is localized to the inner membrane of E. coli (Kaur, P. (1997) J. Bacteriol. 179, 569-575). The present study provides evidence that DrrB, the membrane component of the complex, is stably maintained in the cell only if DrrA is present. Furthermore, it was found that the catalytic component DrrA is in an active conformation only when it is in a complex with DrrB. In a subclone containing the drrB gene by itself, no DrrB protein could be detected, although a translational fusion of the first 15 amino acids of DrrB to beta-galactosidase indicated that DrrB is translated in the absence of DrrA. Upon co-transformation with a plasmid containing the drrA gene in trans, DrrB could again be detected in these cells. UV cross-linking studies with [alpha-32P]ATP showed that only the membrane-bound form of...Continue Reading

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Citations

May 7, 2010·Journal of Industrial Microbiology & Biotechnology·Eric Cundliffe, Arnold L Demain
Feb 15, 2001·Trends in Microbiology·M I Borges-Walmsley, A R Walmsley
Jan 16, 2003·Trends in Microbiology·Kenneth S McKeeganAdrian R Walmsley
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Jan 14, 2004·Drugs·Xian-Zhi Li, Hiroshi Nikaido
Mar 19, 2013·The Journal of Biological Chemistry·Wen LiParjit Kaur
Dec 10, 2008·Journal of Molecular Biology·Prajakta PradhanParjit Kaur
Jan 19, 2011·Microbiological Research·Tianjie YuanYoujia Hu
Feb 28, 2015·Biochemical and Biophysical Research Communications·Han ZhangParjit Kaur
Feb 18, 2010·FEMS Microbiology Letters·Padmanabhan SrinivasanRanjan Prasad
Nov 23, 2016·Molecular Microbiology·Tan-Jun WangYong-Quan Li
Apr 20, 2004·The Journal of Biological Chemistry·Suvarna M GandlurParjit Kaur
Jan 5, 2021·Applied Microbiology and Biotechnology·Xinran WangLinquan Bai
Jun 30, 2019·Research in Microbiology·Cédric OrelleJean-Michel Jault
Jul 21, 2004·Biochemistry·Orsolya PolgarSusan E Bates
Oct 13, 2021·FEMS Microbiology Reviews·Sille RemmMarkus A Seeger

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