PMID: 6168745Sep 1, 1981Paper

Biochemical demonstration of the myelin-associated glycoprotein in the peripheral nervous system

Journal of Neurochemistry
D A FiglewiczN H Sternberger

Abstract

Recent immunocytochemical studies indicated that the myelin-associated glycoprotein (MAG) is localized in the periaxonal region of central nervous system (CNS) and peripheral nervous system (PNS) myelin sheaths but previous biochemical studies had not demonstrated the presence of MAG in peripheral nerve. The glycoproteins in rat sciatic nerves were heavily labeled by injection of [3H]fucose in order to re-examine whether MAG could be detected chemically in peripheral nerve. Myelin and a myelin-related fraction, W1, were isolated from the nerves. Labeled glycoproteins in the PNS fractions were extracted by the lithium diiodosalicylate (LIS)-phenol procedure, and the extracts were treated with antiserum prepared to CNS MAG in a double antibody precipitation. This resulted in the immune precipitation of a single [3H]fucose-labeled glycoprotein with electrophoretic mobility very similar to that of [14C]fucose-labeled MAG from rat brain. A sensitive peptide mapping procedure involving iodination with Bolton-Hunter reagent and autoradiography was used to compare the peptide maps generated by limited proteolysis from this PNS component and CNS MAG. The peptide maps produced by three distinct proteases were virtually identical for the ...Continue Reading

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Citations

Apr 29, 1999·Journal of Neuroscience Research·H YangM Schachner
Jun 1, 1986·Brain Research·T InuzukaR H Quarles
Jul 1, 1984·Journal of Neuroimmunology·B D Trapp, R H Quarles
Apr 1, 1986·Journal of Neuroimmunology·E Nobile-OrazioG Scarlato
Jan 1, 1981·Neurochemistry International·J M Matthieu
Mar 23, 2000·Progress in Neurobiology·B GarbayC Cassagne
Jan 24, 2007·Journal of Neurochemistry·Richard H Quarles
Jan 1, 1991·Acta Neuropathologica·P Baron, B Kreider
Apr 1, 1982·Journal of Neurochemistry·M E Smith, N H Sternberger
Aug 1, 1982·Journal of Neurochemistry·D PleasureS Brown
Nov 1, 1982·Journal of Neurochemistry·P E BraunN Latov
Nov 1, 1982·Journal of Neurochemistry·D JohnsonR O Brady
Jan 1, 1990·Annals of the New York Academy of Sciences·B D Trapp
Jun 6, 2008·Journal of Clinical Neuromuscular Disease·Khema R SharmaWilliam Sheremata
Jun 1, 1988·Cellular and Molecular Neurobiology·R O Brady, R H Quarles
Jan 1, 1993·Muscle & Nerve·S G Waxman
Jun 1, 1984·Annals of Neurology·M C Dalakas, N M Papadopoulos
Jul 1, 1990·Journal of Neuroscience Research·A C LeBlanc, J F Poduslo
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Oct 18, 2020·Alcoholism, Clinical and Experimental Research·Bradley J ChattertonLisa M Savage
Apr 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Christian BüllYoshiki Narimatsu

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