Biochemical detection of type I cell damage after nitrogen dioxide-induced lung injury in rats

The American Journal of Physiology
Mary C McElroyLeland G Dobbs

Abstract

We have previously shown that injury to lung epithelial type I cells can be detected biochemically by measuring the airway fluid content of a type I cell-specific protein, rTI40, in a model of severe acute lung injury [M. C. McElroy, J.-F. Pittet, S. Hashimoto, L. Allen, J. P. Wiener-Kronish, and L. G. Dobbs. Am. J. Physiol. 268 (Lung Cell. Mol. Physiol. 12): L181-L186, 1995]. The first objective of the present study was to evaluate the utility of rTI40 in the assessment of alveolar injury in a model of milder acute lung injury. Rats were exposed to 18 parts/ million NO2 for 12 h; control rats received filtered air for 12 h. In NO2-exposed rats, the total amount of rTI40 in bronchoalveolar fluid was elevated 2-fold compared with control values (P < 0.001); protein concentration was 8.5-fold of control values (P < 0.001). The increase in rTI40 was associated with morphological evidence of injury to type I cells limited to the proximal alveolar regions of the lung. The second objective was to correlate the severity of alveolar type I cell injury with functional measurements of lung epithelial barrier integrity. NO2 inhalation stimulated distal air space fluid clearance despite a significant increase in lung endothelial and epithe...Continue Reading

References

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Citations

May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·G M BoylanM C McElroy
Jun 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Randolph H HastingsLeonard J Deftos
Apr 26, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Gareth R CleggMary C McElroy
Feb 4, 2006·American Journal of Respiratory and Critical Care Medicine·Tokujiro UchidaMichael A Matthay
Nov 13, 2002·Annual Review of Physiology·Mary C Williams

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