PMID: 6411075Jun 15, 1983Paper

Biochemical differences in the mechanism of macrophage lysosomal exocytosis initiated by zymosan particles and weak bases

The Biochemical Journal
D W RichesD R Stanworth

Abstract

By utilizing compounds with different inhibitory properties, discrete biochemical differences were found in the mechanism of selective lysosomal enzyme secretion by macrophages in response to stimulation with zymosan particles and methylamine. Pretreatment of macrophages with trypsin markedly impaired the capacity of the cells to respond to stimulation with zymosan particles, but had no effect on methylamine-stimulated lysosomal enzyme secretion. Similarly, the addition of phenylmethanesulphonyl fluoride or EDTA to the incubation medium substantially inhibited zymosan-induced lysosomal enzyme secretion, whereas the methylamine-stimulated response was unaffected by these agents. The addition of 2-deoxyglucose to incubation media, however, strongly inhibited both zymosan- and methylamine-stimulated beta-galactosidase secretion. These findings are consistent with a mechanism for lysosomal enzyme secretion by macrophages, based on a receptor-dependent uptake of zymosan particles and a receptor-independent uptake of methylamine.

Citations

May 19, 1998·Pigment Cell Research·R T SwankL Feng
Nov 13, 2012·Biochemical and Biophysical Research Communications·Youn Ho ShinJunyang Jung
Feb 1, 1984·Bioscience Reports·N Latif, B K Bachhawat
Dec 15, 2012·Biochemical and Biophysical Research Communications·Junyang JungHiroshi Kiyama
Dec 24, 2013·Cellular and Molecular Neurobiology·Youn Ho ShinNa Young Jeong

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