Biochemical differentiation of mechanically dissociated mammalian brain in aggregating cell culture
Mouse and rat brain cells were dissociated by a simple mechanical sieving technique and studied in culture for the formation of aggregates and the activities of choline acetyltransferase, acetylcholinesterase, glutamic acid decarboxylase, tyrosine 3-monooxygenase, aromatic L-amino acid decarboxylase, catechol methyltransferase, and monoamine oxidase. Cells from fetal and neonatal tissue formed aggregates but not cells from tissue older than two days after birth. The pattern of development of enzyme activities in these aggregates varied with the age of starting tissue. The highest levels of specific activity for the neuron-specific enzymes were found after 3-4 weeks in culture for aggregates of cells derived from relatively undeveloped brains.
Surface culture of fetal mammalian brain cells: effect of subculture on morphology and choline acetyltransferase activity
A study on the mechanism of intercellular adhesion. Effects of neuraminidase, calcium, and trypsin on the aggregation of suspended HeLa cells
Cholinesterase and choline acetyltransferase activities develop in whole explant but not in dissociated cell cultures of cockroach brain
Rotation-mediated histogenetic aggregation of dissociated cells. A quantifiable approach to cell interactions in vitro
Patients with active relapsing-remitting multiple sclerosis synthesize antibodies recognizing oligodendrocyte progenitor cell surface protein: implications for remyelination
Lack of desensitization of muscarinic receptor-mediated second messenger signals in rat brain upon acute and chronic inhibition of acetylcholinesterase
Insulin-like growth factor I stimulates oligodendrocyte development and myelination in rat brain aggregate cultures
Role for glial cells in regulating the functional expression of neuropeptide Y (NPY) neurons in aggregate cultures derived from dissociated fetal brain cells
Quantitative analysis of cancer invasion in vitro: comparison of two new assays and of tumour sublines with different metastatic capacity
High-affinity [3H]inositol uptake by dissociated brain cells and cultured fibroblasts from fetal mice
Ultrastructural investigation of fetal rat brain hemisphere tissue in nonadherent stationary organ culture
Neurotensin and its analogs--correlation of specific binding with stimulation of cyclic GMP formation in neuroblastoma clone N1E-115
Desensitization of neurotensin receptor-mediated cyclic GMP formation in neuroblastoma clone N1E-115
Morphological differentiation of mechanically dissociated fetal rat brain in aggregating cell cultures
Methionine-enkephalin immunoreactivity in fetal rat brain cells in aggregating culture and in mouse neuroblastoma cells
Differentiation of neuron-like cells in cultured rat optic nerves: a neuron or common neuron-glia progenitor?
Antidepressants are weak competitive antagonists of histamine H2 receptors in dissociated brain tissue
Non-selectivity of amitriptyline for subtypes of brain muscarinic receptors demonstrated in binding and functional assays
Permeability of the blood brain barrier by the bradykinin agonist, RMP-7: evidence for a sensitive, auto-regulated, receptor-mediated system
Nerve growth factor (NGF) stimulation of cholinergic telencephalic neurons in aggregating cell cultures
Epidermal growth factor (EGF) stimulation of cultured brain cells. I. Enhancement of the developmental increase in glial enzymatic activity
Epidermal growth factor (EGF) stimulation of cultured brain cells. II. Increased production of extracellular soluble proteins
Epidermal growth factor and bovine growth hormone stimulate differentiation and myelination of brain cell aggregates in culture
Cholinergic neurons of fetal rat telencephalon in aggregating cell culture respond to NGF as well as to protein kinase C-activating tumor promoters
Use of aggregating brain cultures to study the replication of herpes simplex virus types 1 and 2 in central nervous system tissue
Enhancement by estrogen treatment of -bungarotoxin binding in fetal mouse amygdala cells reaggregated in vitro
Aging enhances the calcium sensitivity of central neurons of the mouse as an adaptive response to reduced free intracellular calcium
Cells with neuronal characteristics differentiate and persist for one year in rat optic nerve explants cultures
Some electrophysiological properties of cultured rat cerebral cortical neurons dissociated from fetuses at various gestational ages
Expression of the three opioid receptor subtypes mu, delta and kappa in guinea pig and rat brain cell cultures and in vivo
The acute and subchronic effects of organophosphorus and carbamate pesticides on cholinesterase activity in aggregate cultures of neural cells from the foetal rat brain
Developmental regulation of neurotensin receptor expression and function in murine neuroblastoma clone N1E-115
Preliminary characterisation of an in vitro paradigm for the study of the delayed effects of organophosphorus compounds: hen embryo brain spheroids
An improved method for dissociation and aggregate culture of human fetal brain cells in serum-free medium
Involvement of calcium channels in short-term desensitization of muscarinic receptor-mediated cyclic GMP formation in mouse neuroblastoma cells
Stop-flow analysis of the axonal transport of dopa decarboxylase (EC 126.96.36.199) in rabbit sciatic nerves
Reduction of muscarinic receptor density and of guanine nucleotide-stimulated phosphoinositide hydrolysis in human SH-SY5Y neuroblastoma cells following long-term treatment with 12-O-tetradecanoylphorbol 13-acetate or mezerein
Identification of multiple phosphoinositide-linked receptors on human SK-N-MC neuroepithelioma cells
Comparative metabolism of fluorinated 3,4-dihydroxyphenylalanine isomers in aggregating brain cell cultures
Contribution of G protein activation to fluoride stimulation of phosphoinositide hydrolysis in human neuroblastoma cells
Increase in choline acetyltransferase activity in surgically isolated postganglionic parasympathetic neurones of the urinary bladder of adult rats
Characterization of long-term mouse brain aggregating cultures: evidence for maintenance of neural precursor cells
Differentiation of cells in aggregates from human, mouse, and chick embryo brain. An electronmicroscopic study
Cannabinoid-mediated neuroprotection following interferon-gamma treatment in a three-dimensional mouse brain aggregate cell culture
An in vitro model for de- and remyelination using lysophosphatidyl choline in rodent whole brain spheroid cultures
Enhanced neuronal expression in reaggregating cells of mouse cerebellum cultured in the presence of poly-L-lysine
Glial fibrillary acidic protein in reaggregating and monolayer cultures of fetal mouse cerebral hemispheres
Astrocytes, ependymal cells, and oligodendrocytes develop on schedule in dissociated cell cultures of embryonic rat brain
Tracing the development of oligodendrocytes from precursor cells using monoclonal antibodies, fluorescence-activated cell sorting, and cell culture
The transition from embryonic to adult isozyme expression in reaggregating cell cultures of mouse brain
Inhibition of cyclic AMP formation in N1E-115 neuroblastoma cells is mediated by a non-cardiac M2 muscarinic receptor subtype
Mixed competitive and allosteric antagonism by gallamine of muscarinic receptor-mediated second messenger responses in N1E-115 neuroblastoma cells
Cyclic AMP induction of the myelin enzyme 2',3'-cyclic nucleotide 3'-phosphohydrolase in rat oligodendrocytes
Protein kinase C-activating tumor promoters enhance the differentiation of astrocytes in aggregating fetal brain cell cultures
Influence of delta-opioid receptors on production of labeled methionine5-enkephalin in murine neuroblastoma cells
Regulation of muscarinic receptor-mediated cyclic GMP synthesis by cultured mouse neuroblastoma cells
Development of markers for cholinergic neurones in re-aggregate cultures of foetal rat whole brain in serum-containing and serum-free media: effects of triiodothyronine (T3)
Temperature dependence of muscarinic acetylcholine receptor activation, desensitization, and resensitization
Differential sensitivity of phosphoinositide and cyclic GMP responses to short-term regulation by a muscarinic agonist in mouse neuroblastoma cells. Correlation with down-regulation of cell surface receptors
Patterns of reaggregation and formation of linear aggregate chains in collagen-well cultures of dissociated mouse brain and spinal cord cells
Activation of muscarinic cholinergic receptors enhances the volume-sensitive efflux of myo-inositol from SH-SY5Y neuroblastoma cells
Muscarinic receptor-stimulated phosphoinositide turnover in human SK-N-SH neuroblastoma cells: differential inhibition by agents that elevate cyclic AMP
Glucocorticoids potentiate the prostaglandin E1-mediated cyclic AMP formation by a cultured murine neuroblastoma clone
Biochemical characterization of [3H]norepinephrine uptake in dissociated brain cell cultures from chick embryos
Stirred vessel cultures of rat brain cells aggregates: characterization of major metabolic pathways and cell population dynamics
Genistein-induced neuronal differentiation is associated with activation of extracellular signal-regulated kinases and upregulation of p21 and N-cadherin
Potentiation of the osmosensitive release of taurine and D-aspartate from SH-SY5Y neuroblastoma cells after activation of M3 muscarinic cholinergic receptors
Characterization of three-dimensional rat central nervous system culture maturation, with applications to monitor cholinergic integrity
The dynamics of cGMP metabolism in neuroblastoma N1E-115 cells determined by 18O labeling of guanine nucleotide alpha-phosphoryls
Advances in 3D neural, vascular and neurovascular models for drug testing and regenerative medicine.
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