Abstract
Marine plants and animals are sources of a huge number of pharmacologically active compounds, some of which exhibit antineoplastic activity of clinical relevance. However the mechanism of action of marine natural products (MNPs) is poorly understood. In this study, proton NMR spectroscopy-based metabolomics was applied to unravel biochemical disorders induced in human MCF7 breast cancer cells by 3 lead candidate anticancer MNPs: ascididemin (Asc), lamellarin-D (Lam-D), and kahalalide F (KF). Asc, Lam-D, and KF provoked a severe decrease in DNA content in MCF7 cells after 24-h treatment. Asc and Lam-D provoked apoptosis, whereas KF induced non-apoptotic cell death. Metabolite profiling revealed major biochemical disorders following treatment. The response of MCF7 tumor cells to Asc involved the accumulation of citrate (x17 the control level, P<0.001), testifying enzyme blockade in citrate metabolism, and the accumulation of gluconate (x9.8, P<0.005), a metabolite never reported at such concentration in tumor cells, probably testifying glycolysis shutdown. The response to Lam-D involved the accumulation of aspartate (x7.2, P<0.05), glutamate (x14.7, P<0.05), and lactate (x2.3, P<0.05), probably in relation with the targeting of t...Continue Reading
References
Nov 15, 1990·Analytical Biochemistry·R RagoG Wilding
Jul 1, 1995·Brain Research. Molecular Brain Research·V Mehta, M A Namboodiri
Jan 19, 1996·Cancer Letters·M García-RochaJ Avila
Mar 15, 1996·Biochimica Et Biophysica Acta·P MendesH V Westerhoff
Jan 23, 1999·The Journal of Biological Chemistry·F SalveminiM V Ursini
Jun 30, 2000·Biochemical Pharmacology·L DassonnevilleC Bailly
Feb 18, 2003·Chemical Research in Toxicology·Sandra S MatsumotoLouis R Barrows
Oct 22, 2003·Circulation·Dirk LebrechtUlrich A Walker
Jan 13, 2004·Oncogene·Verena M DirschAngelika M Vollmar
Jul 2, 2004·Nature Reviews. Cancer·Julian L Griffin, John P Shockcor
Mar 10, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jeany M Rademaker-LakhaiJan H M Schellens
Apr 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Arvind RamanathanStuart L Schreiber
May 24, 2005·Molecular Pharmacology·Maarten L JanmaatGiuseppe Giaccone
Jun 15, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J M SewellS M Guichard
Mar 17, 2006·Cancer Research·Jérôme KluzaChristian Bailly
Mar 23, 2006·The Journal of Biological Chemistry·Kiyotaka MachidaHiroyuki Osada
Apr 7, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Daniel MorvanJean Claude Madelmont
Jun 3, 2006·Journal of Proteome Research·E HolmesJ K Nicholson
Oct 28, 2006·Journal of Natural Products·Sonia ManzanaroJesús Angel de la Fuente
Jan 24, 2007·Physiological Reviews·Jorgina SatrústeguiAraceli Del Arco
Mar 3, 2007·Cancer Research·Daniel Morvan, Aicha Demidem
Oct 2, 2007·Oncogene·M-A GallegoP Marchetti
Dec 22, 2007·Chemical Reviews·Hui FanJin-Feng Hu
Jan 9, 2008·Apoptosis : an International Journal on Programmed Cell Death·Christoffer TammSandra Ceccatelli
Feb 28, 2008·Journal of Natural Products·Sanjeev BanerjeeRamzi M Mohammad
Jul 30, 2008·Cell Research·Iyoko KatohShun-Ichi Kurata
Jan 9, 2009·The Journal of Biological Chemistry·Laura Contreras, Jorgina Satrústegui
Jan 28, 2009·Marine Drugs·Dianne BaunbaekLaurent Meijer
Feb 3, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Salvador Martín-AlgarraLuis Paz-Ares
Jun 11, 2009·Breast Cancer Research and Treatment·Mathilde Bayet-RobertChantal Barthomeuf
Dec 3, 2009·Molecular Cancer Therapeutics·Caroline BallotPhilippe Marchetti
Feb 13, 2010·Apoptosis : an International Journal on Programmed Cell Death·Caroline BallotPhilippe Marchetti
May 1, 2010·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Mathilde Bayet-RobertDaniel Morvan
Citations
May 18, 2013·Chemical Reviews·Sandip B BharateRam A Vishwakarma
Mar 9, 2013·PloS One·Mathilde Bayet-Robert, Daniel Morvan
Oct 25, 2013·Marine Drugs·Daniel Morvan
Dec 13, 2012·Oncology Reports·Shinsuke SasadaMorihito Okada
Feb 24, 2015·Marine Drugs·Christian Bailly
Dec 23, 2014·Marine Drugs·Dennis ImbriTill Opatz
Oct 16, 2012·Journal of Inorganic Biochemistry·Altea RocchiGianni Valensin
Oct 15, 2013·Chemistry : a European Journal·Dennis ImbriTill Opatz
Jun 14, 2016·Journal of Medicinal Chemistry·Michel FrédérichPascal de Tullio
Jan 1, 2013·Metabolites·Marie S A Palmnas, Hans J Vogel
Feb 7, 2020·Expert Opinion on Drug Discovery·Kevin Andrew StuartRuAngelie Edrada-Ebel
May 2, 2017·Briefings in Bioinformatics·Jiansong FangFeixiong Cheng
Sep 26, 2013·Molecular Medicine Reports·Xiaowei XuXiangjun Meng