Biochemical diversity in the Trypanosoma congolense trans-sialidase family

PLoS Neglected Tropical Diseases
Thaddeus T GbemSørge Kelm

Abstract

Trans-sialidases are key enzymes in the life cycle of African trypanosomes in both, mammalian host and insect vector and have been associated with the disease trypanosomiasis, namely sleeping sickness and nagana. Besides the previously reported TconTS1, we have identified three additional active trans-sialidases, TconTS2, TconTS3 and TconTS4, and three trans-sialidase like genes in Trypanosoma congolense. At least TconTS1, TconTS2 and TconTS4 are found in the bloodstream of infected animals. We have characterised the enzymatic properties of recombinant proteins expressed in eukaryotic fibroblasts using fetuin as model blood glycoprotein donor substrate. One of the recombinant trans-sialidases, TconTS2, had the highest specific activity reported thus far with very low sialidase activity. The active trans-sialidases share all the amino acids critical for the catalytic reaction with few variations in the predicted binding site for the leaving or acceptor glycan. However, these differences cannot explain the orders of magnitudes between their transfer activities, which must be due to other unidentified structural features of the proteins or substrates selectivity. Interestingly, the phylogenetic relationships between the lectin dom...Continue Reading

References

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Mar 22, 2003·The Journal of Biological Chemistry·Evelin TiralongoRoland Schauer
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Sep 8, 2006·The Journal of Biological Chemistry·Georgina N MontagnaAlberto C C Frasch
Feb 16, 2008·Parasites & Vectors·Dietmar Steverding
Aug 2, 2011·BMC Biochemistry·Hendrik Koliwer-BrandlSørge Kelm
Nov 2, 2011·Biochemical and Biophysical Research Communications·Fumiki NakataniTaroh Kinoshita
Mar 21, 2012·Infection and Immunity·Luis Miguel De Pablos, Antonio Osuna
Jul 4, 2012·Cellular Microbiology·Sergio S C Dc-Rubin, Sergio Schenkman
Apr 5, 2013·PLoS Neglected Tropical Diseases·Andrew P JacksonMatthew Berriman

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Citations

Oct 17, 2015·PLoS Neglected Tropical Diseases·Mario WaespySørge Kelm
Jan 24, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Oscar CampetellaMaría Susana Leguizamón

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Datasets Mentioned

BETA
AJ535487
AJ535488

Methods Mentioned

BETA
transfection
X-ray
PCR
FCS
transgenic

Software Mentioned

Contig Express
MrBayes
Geneious
MEGA5
Like
ClustalW
SigmapPlot
RevTrans
SigmaPlot
Basic Local Alignment Search Tool ( BLAST )

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