Biochemical evidence for the differential association of metabotropic glutamate receptors within synaptic complexes

Neuroscience Letters
Ayodeji A AsuniVincent O'Connor

Abstract

The distribution of metabotropic glutamate (mGlu) receptors within the synapse is an important determinant of function. mGlu have been grouped together into three main sub-classes: Group I mGlu (1 and 5) are predominantly situated on the post-synaptic membrane, whereas Group III (4, 6, 7 and 8) are largely pre-synaptic. Group II mGlu (2 and 3) are distributed peripheral to the active zone, on both sides of the synaptic cleft. Methods based on a distinct pH-dependent extractability of the pre- and post-synaptic marker proteins can provide insight into the molecular organization of synaptic junctions [G.R. Phillips, J.K. Huang, Y. Wang, H. Tanaka, L. Shapiro, W. Zhang, W. Shan, K. Arndt, M. Frank, R.E. Gordon, M.A. Gawinowicz, Y. Zhao and D.R. Colman, The presynaptic particle web: ultrastructure, composition, dissolution and reconstitution, Neuron 32 (2001) 63-77]. We have applied such procedures to rat brain cortical synaptosomes to explore the biochemical evidence for the accepted localisations of metabotropic glutamate receptors. As shown previously a number of post-synaptic marker proteins remained detergent-insoluble at both pH 6 and pH 8. There was an increased extraction of a number of pre-synaptic plasma membrane and cyto...Continue Reading

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Citations

May 20, 2011·Journal of Neurochemistry·Joanne L BaileyJohn E Chad

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