Biochemical, genetic and transcriptional characterization of multibacteriocin production by the anti-pneumococcal dairy strain Streptococcus infantarius LP90

PloS One
Cristina CampaneroL M Cintas

Abstract

Streptococcus pneumoniae infections are one of the major causes of morbility and mortality worldwide. Although vaccination and antibiotherapy constitute fundamental and complementary strategies against pneumococcal infections, they present some limitations including the increase in non-vaccine serotypes and the emergence of multidrug-resistances, respectively. Ribosomally-synthesized antimicrobial peptides (i.e. bacteriocins) produced by Lactic Acid Bacteria (LAB) may represent an alternative or complementary strategy to antibiotics for the control of pneumococal infections. We tested the antimicrobial activity of 37 bacteriocinogenic LAB, isolated from food and other sources, against clinical S. pneumoniae strains. Streptococcus infantarius subsp. infantarius LP90, isolated from Venezuelan water-buffalo milk, was selected because of its broad and strong anti-pneumococcal spectrum. The in vitro safety assessment of S. infantarius LP90 revealed that it may be considered avirulent. The analysis of a 19,539-bp cluster showed the presence of 29 putative open reading frames (ORFs), including the genes encoding 8 new class II-bacteriocins, as well as the proteins involved in their secretion, immunity and regulation. Transcriptional a...Continue Reading

References

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Citations

Jan 5, 2021·World Journal of Microbiology & Biotechnology·Laura García-CurielNorberto Chavarría-Hernández
Aug 4, 2021·Probiotics and Antimicrobial Proteins·Lorena Trejo-GonzálezNorberto Chavarría-Hernández

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Datasets Mentioned

BETA
BAA-102

Methods Mentioned

BETA
PCR
electrophoresis
amino acid sequencing
gel filtration
Protein Liquid
Protein Sequencing

Software Mentioned

BLAST
ORF finder
ARNold
ExPASy

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