Biochemical identification and tissue-specific expression patterns of keratins in the zebrafish Danio rerio

Cell and Tissue Research
Matthias ConradJürgen Markl

Abstract

We have identified a number of type I and type II keratins in the zebrafish Danio rerio by two-dimensional polyacrylamide gel electrophoresis, complementary keratin blot-binding assay and immunoblotting. These keratins range from 56 kDa to 46 kDa in molecular mass and from pH 6.6 to pH 5.2 in isoelectric point. Type II zebrafish keratins exhibit significantly higher molecular masses (56-52 kDa) compared with the type I keratins (50-48 kDa), but the isoelectric points show no significant difference between the two keratin subclasses (type II: pH 6.0-5.5; type I: pH 6.1-5.2). According to their occurrence in various zebrafish tissues, the identified keratins can be classified into "E" (epidermal) and "S" (simple epithelial) proteins. A panel of monoclonal anti-keratin antibodies has been used for immunoblotting of zebrafish cytoskeletal preparations and immunofluorescence microscopy of frozen tissue sections. These antibodies have revealed differential cytoplasmic expression of keratins; this not only includes epithelia, but also a variety of mesenchymally derived cells and tissues. Thus, previously detected fundamental differences in keratin expression patterns between higher vertebrates and a salmonid, the rainbow trout Oncorhy...Continue Reading

Citations

May 23, 2002·Developmental Cell·Hyunsook Lee, David Kimelman
Jun 8, 2007·Frontiers in Zoology·Michael SchaffeldChristian Hunzinger
Jan 25, 2008·Current Eye Research·Chun-Chen ChenI-Jong Wang
Dec 25, 2012·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Bradley L BaumgarnerPaul B Brown
Dec 22, 1998·European Journal of Cell Biology·J CerdàH Herrmann
Dec 11, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P-E MaugerP-Y Le Bail
Dec 16, 2006·Zoology : Analysis of Complex Systems, ZACS·Mattia Toni, Lorenzo Alibardi
Oct 28, 2015·Diseases of Aquatic Organisms·Colleen E PaquetteChristiane V Löhr
Sep 18, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Bhaja K PadhiMarie-Andrée Akimenko
Dec 4, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chung-Der HsiaoHuai-Jen Tsai
Aug 25, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Angèle Tingaud-SequeiraPatrick J Babin
Sep 12, 2006·Zoology : Analysis of Complex Systems, ZACS·Debasish Das, Tapas C Nag
Apr 12, 2005·European Journal of Cell Biology·Michael SchaffeldJürgen Markl
Sep 21, 2000·Differentiation; Research in Biological Diversity·K L Chua, T M Lim
Feb 1, 2003·Differentiation; Research in Biological Diversity·Michael SchaffeldJürgen Markl
Aug 23, 2002·Differentiation; Research in Biological Diversity·Michael SchaffeldJürgen Markl
Aug 23, 2002·Differentiation; Research in Biological Diversity·Michael SchaffeldJürgen Markl
Sep 6, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Luis D NeveDana M García
Feb 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Zhiyuan GongTie Yan
Mar 5, 2003·Journal of Morphology·Lorenzo Alibardi, Jean M P Joss
Jul 3, 2021·Polymers·Simona Perța-CrișanFlorentina-Daniela Munteanu
Dec 24, 2018·Journal of Proteome Research·Rebecca J PawlukSofia Consuegra
Sep 29, 2019·Molecular Immunology·Ko ShibuyaOsamu Nakamura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved