PMID: 6407031Apr 1, 1983Paper

Biochemical interaction of arachidonic acid and vitamin E in human platelets

Prostaglandins, Leukotrienes, and Medicine
R Mower, M Steiner

Abstract

The effect of alpha-tocopherol on the oxidative transformation of arachidonic acid was investigated in human platelets. The major products of lipoxygenase and cyclo-oxygenase pathways were separated by high performance liquid chromatography (HPLC) and thin layer chromatography (TLC) evaluated by scanning the radiochromatograms. This study differs from others in the vitamin E field in important aspects of its experimental design: the prelabeling of platelets with non-aggregating concentrations of 14C-arachidonic acid, and the addition of alpha-tocopherol as a colloidal suspension rather than as an ethanolic solution. A moderately potent but consistent reduction of apparent cyclo-oxygenase activity by alpha-tocopherol could be demonstrated by TLC and HPLC. This effect was best shown by the change of the HETE/HHT ratio which increased significantly in vitamin E-treated platelets. It was found to be dose-dependent up to 1 microM alpha-tocopherol, the maximal concentration tested in this study. Alpha-tocopherol quinone was equally effective in this action.

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Citations

Jan 1, 1987·The International Journal of Biochemistry·E MalleG M Kostner
Oct 24, 1998·Prostaglandins & Other Lipid Mediators·H JohnW Schlegel
Oct 1, 1991·Journal of the American College of Nutrition·M Steiner
Sep 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P K DuitsmanS Hendrich
May 15, 1988·Thrombosis Research·N HuangM Steiner
Jan 15, 2014·Global Advances in Health and Medicine : Improving Healthcare Outcomes Worldwide·Gunver S KienleHelmut Kiene
Jul 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·C GisingerJ A Colwell

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