Biochemical issues in estimation of cytosolic free NAD/NADH ratio.

PloS One
Feifei SunXun Hu

Abstract

Cytosolic free NAD/NADH ratio is fundamentally important in maintaining cellular redox homeostasis but current techniques cannot distinguish between protein-bound and free NAD/NADH. Williamson et al reported a method to estimate this ratio by cytosolic lactate/pyruvate (L/P) based on the principle of chemical equilibrium. Numerous studies used L/P ratio to estimate the cytosolic free NAD/NADH ratio by assuming that the conversion in cells was at near-equilibrium but not verifying how near it was. In addition, it seems accepted that cytosolic free NAD/NADH ratio was a dependent variable responding to the change of L/P ratio. In this study, we show (1) that the change of lactate/glucose (percentage of glucose that converts to lactate by cells) and L/P ratio could measure the status of conversion between pyruvate + NADH and lactate + NAD that tends to or gets away from equilibrium; (2) that cytosolic free NAD/NADH could be accurately estimated by L/P only when the conversion is at or very close to equilibrium otherwise a calculation error by one order of magnitude could be introduced; (3) that cytosolic free NAD/NADH is stable and L/P is highly labile, that the highly labile L/P is crucial to maintain the homeostasis of NAD/NADH; ...Continue Reading

References

Mar 1, 1976·Metabolism: Clinical and Experimental·A I Arieff, A Kerian
Jul 1, 1976·Journal of Neurochemistry·G E Gibson, J P Blass
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D L RothmanR G Shulman
Jan 1, 1988·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·N NittaK Ozawa
Jan 1, 1985·The International Journal of Biochemistry·J M SiverioE Meléndez-Hevia
Jan 1, 1974·Connective Tissue Research·K MurataT Okuyama
Jul 1, 1965·Archives of Biochemistry and Biophysics·B M AndersonC D Anderson
Mar 1, 1974·Journal of Neurochemistry·H S BachelardB K Siesjö
Jan 1, 1981·European Journal of Applied Physiology and Occupational Physiology·H A Davis, G C Cass
Jun 1, 1993·Diabetes·J R WilliamsonR G Tilton
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·T S YongS L Stanley
Oct 1, 1996·Journal of Bacteriology·M R LeonardoD P Clark
Apr 18, 1998·Molecular and Cellular Biochemistry·T C VaryS R Kimball
Aug 6, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·I G ObrosovaD A Greene
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·E BassengeR Bünger
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J T BarronJ E Parrillo
Jan 12, 2001·FEMS Microbiology Reviews·B M BakkerJ T Pronk
Nov 16, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Irina G ObrosovaMartin J Stevens
Jan 5, 2002·Physiological Reviews·Wulf Dröge
Mar 9, 2002·The Journal of Biological Chemistry·Rozalyn M AndersonDavid A Sinclair
Jun 5, 2002·Journal of Hepatology·Saul J Karpen
Sep 7, 2002·Molecular and Cellular Biology·Anna N MoraitisCatherine C Thompson
Mar 22, 2003·Current Opinion in Cell Biology·Su-Ju Lin, Leonard Guarente
Aug 10, 1956·Science·O WARBURG
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yasuo IdoJoseph R Williamson
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mark A MintunMarcus E Raichle
Jan 16, 2004·Genes & Development·Su-Ju LinLeonard Guarente
Mar 9, 2004·Trends in Biochemical Sciences·Felicitas BergerMathias Ziegler
Nov 2, 2004·Nature Reviews. Cancer·Robert A Gatenby, Robert J Gillies
Sep 15, 2005·The FEBS Journal·Mathias Ziegler

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Citations

Jan 30, 2013·Molecular Aspects of Medicine·Carles CantóPeter Bai
Sep 17, 2015·Journal of Proteome Research·Lixiao GuHaiteng Deng
May 14, 2014·Scientific Reports·Jiansheng XieXun Hu
Jun 12, 2013·Transfusion·Walter Sunny DzikChristopher Stowell
Apr 21, 2016·Archives of Biochemistry and Biophysics·Jose ViñaConsuelo Borrás
Feb 12, 2014·The International Journal of Biochemistry & Cell Biology·Rafael Moreno-SánchezSara Rodríguez-Enríquez
May 20, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Halina FalfushynskaRostyslav Stoika
Dec 17, 2014·Frontiers in Neuroscience·Daniel A Kane
Apr 14, 2016·PloS One·Magdalena Orczyk-PawilowiczPiotr Mlynarz
May 4, 2017·Journal of the American Chemical Society·Jae Mo ParkKelvin L Billingsley
Mar 7, 2017·Archives of Biochemistry and Biophysics·Andrew N Lane, Teresa W-M Fan
Dec 13, 2016·Trends in Neurosciences·John Darrell Van HornAndrei Irimia
Jun 22, 2017·Antioxidants & Redox Signaling·Joshua P Fessel, William M Oldham
Jan 10, 2019·IUBMB Life·Richard L VeechWilliam Curtis
Nov 2, 2013·The Journal of Biological Chemistry·Valérie Desquiret-DumasVincent Procaccio
Jun 19, 2015·Physiological Reviews·Vidar GundersenLinda Hildegard Bergersen
Sep 2, 2016·Physiological Reviews·David G Nicholls
Jan 31, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keely PierzchalskiMaureen A Kane
Apr 12, 2020·Experimental Biology and Medicine·Hang YangZhaocai Zhang
Jul 10, 2016·Journal of Physiology and Biochemistry·Chia George Hsu, Thomas J Burkholder
Nov 7, 2015·Bioscience Reports·J William O Ballard, Neil A Youngson
Feb 26, 2016·Plant Biotechnology Journal·Xiu-Ming WuXiao-Ya Chen
Mar 7, 2017·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jae Mo ParkDaniel M Spielman
Mar 31, 2018·Frontiers in Endocrinology·Isabel Méndez, Mauricio Díaz-Muñoz
Dec 15, 2016·Frontiers in Neuroscience·Stephen A BroseMikhail Y Golovko
Sep 21, 2018·Frontiers in Microbiology·Sébastien Bontemps-GalloFrank C Gherardini
Nov 24, 2016·Frontiers in Cellular and Infection Microbiology·Shabir A BhatAshwani Kumar
Dec 6, 2017·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Eric A F HerbstDaniel A Kane
Aug 1, 2013·American Journal of Physiology. Endocrinology and Metabolism·Xu XuDouglas R Cavener

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