Oct 3, 1977

Biochemical markers of the progress of differentiation in cloned teratocarcinoma cell lines

European Journal of Biochemistry
E D AdamsonG G Magrane


The progeny of single teratocarcinoma cells will give rise to several different cell types in vitro, and the latter were shown to be functionally differentiated by biochemical criteria. In all these studies, cloned lines of mouse teratocarcinoma cells were assayed during the course of differentiation for some biochemical products characteristic of the tissues formed. The carcinoembryonic protein, alpha-foetoprotein, was not synthesized by undifferentiated embryonal carcinoma (EC) cells, but was synthesized in increasing amounts during their differentiation to endoderm-type cells in suspension culture. alpha-Foetoprotein was shown to be a product of endoderm cells, but not all endoderm cells synthesized this protein. During the course of further differentiation when EC cells or aggregates were grown in tissue-culture dishes, other biochemical products appeared. In cultures containing predominantly nerve-type cells, there was a 30-fold increase in the specific activity of acetylcholinesterase, with concomitant appearance of the aldolase isoenzyme characteristic of mouse brain. In some cultures, a small amount of muscle-type cell formation was marked by the appearance of the MB isoenzyme of creatine phosphokinase. Generally, bioch...Continue Reading

  • References19
  • Citations52
  • References19
  • Citations52


Mentioned in this Paper

Macro-Creatine Kinase
Fetal Proteins
Suspension Culture
Embryonal Carcinoma
Creatine Kinase Measurement
Cell Differentiation Process
CNS - Brain (Mmhcc)
Mature Teratoma

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