Biochemical mechanism on GABA accumulation during fruit development in tomato

Plant & Cell Physiology
Takashi AkihiroHiroshi Ezura

Abstract

A large amount of gamma-aminobutyric acid (GABA) was found to accumulate in tomato (Solanum lycopersicum) fruits before the breaker stage. Shortly thereafter, GABA was rapidly catabolized after the breaker stage. We screened the GABA-rich tomato cultivar 'DG03-9' which did not show rapid GABA catabolism after the breaker stage. Although GABA hyperaccumulation and rapid catabolism in fruits is well known, the mechanisms are not clearly understood. In order to clarify these mechanisms, we performed comparative studies of 'Micro-Tom' and 'DG03-9' fruits for the analysis of gene expression levels, protein levels and enzymatic activity levels of GABA biosynthesis- and catabolism-related enzymes. During GABA accumulation, we found positive correlations among GABA contents and expression levels of SlGAD2 and SlGAD3. Both of these genes encode glutamate decarboxylase (GAD) which is a key enzyme of GABA biosynthesis. During GABA catabolism, we found a strong correlation between GABA contents and enzyme activity of alpha-ketoglutarate-dependent GABA transaminase (GABA-TK). The contents of glutamate and aspartate, which are synthesized from GABA and glutamate, respectively, increased with elevation of GABA-TK enzymatic activity. GABA-TK i...Continue Reading

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Citations

Oct 31, 2009·Amino Acids·Augusto SorrequietaEstela M Valle
Jun 27, 2012·Applied Biochemistry and Biotechnology·Claudius Marondedze, Ludivine A Thomas
Jan 10, 2012·Journal of Agricultural and Food Chemistry·Nobukazu MaeHiroshi Ezura
Mar 25, 2014·Journal of Experimental Botany·Adriana BastíasJosé A Casaretto
Dec 14, 2011·Plant & Cell Physiology·Keiichi Mochida, Kazuo Shinozaki
Mar 5, 2016·Plant Science : an International Journal of Experimental Plant Biology·Geoffrey B LumGale G Bozzo
Jan 1, 2016·Plant & Cell Physiology·Masahito ShikataHiroshi Ezura
Oct 17, 2015·Genes & Nutrition·Hannah-Sophie BraunCarolin Deiner
Nov 6, 2015·Cellular & Molecular Biology Letters·Ewa JawieńPiotr Młynarz
Oct 4, 2015·Journal of Plant Physiology·Gizem Dimlioğluİsmail Türkan
Jan 21, 2015·The Plant Journal : for Cell and Molecular Biology·Christopher J SnowdenLee J Sweetlove
Sep 1, 2015·Frontiers in Plant Science·Mariko Takayama, Hiroshi Ezura
Dec 3, 2014·Food Chemistry·Rocio I LimónJuana Frias
Sep 30, 2016·Scientific Reports·Lihong LiuQiaomei Wang
Nov 14, 2016·Cellular and Molecular Life Sciences : CMLS·Sunita A RameshBo Xu
May 12, 2018·Nutrients·Matteo BriguglioMauro Porta
Dec 22, 2019·International Journal of Molecular Sciences·Lei ZhangYongping Cai

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