Biochemical pH clamp: the forgotten resource in membrane bioenergetics

The New Phytologist
Lars H Wegner, Sergey Shabala

Abstract

Solute uptake and release by plant cells are frequently energized by coupling to H+ influx supported by the proton motive force (pmf). The pmf results from a stable pH difference between the apoplast and the cytosol, with bulk values ranging from 4.9 to 5.8 and from 7.1 to 7.5, respectively, in combination with a negative electrical membrane potential. The P-type H+ ATPases pumping H+ from the cytosol into the apoplast at the expense of ATP hydrolysis are generally viewed as the only pmf source, exclusively linking membrane transport to energy metabolism. However, recent evidence suggests that pump activity may be insufficient to energize transport, particularly under stress conditions. Indeed, cytosolic H+ scavenging and apoplastic H+ generation by metabolism (denoted as 'active' buffering in contrast to the readily exhausted 'passive' matrix buffering) also stabilize the pH gradient. In the cytosol, H+ scavenging is mainly associated with malate decarboxylation catalyzed by malic enzyme, and via the GABA shunt of the tricarboxylic acid (TCA) cycle involving glutamate decarboxylation. In the apoplast, formation of bicarbonate from CO2 , the end-product of respiration, generates H+ at pH ≥ 6. Membrane potential is stabilized by...Continue Reading

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T L LomaxW R Briggs
Oct 11, 1996·The Journal of Biological Chemistry·K J BoorerE M Wright
Oct 26, 1999·Trends in Plant Science·B J ShelpM D McLean
Feb 29, 2000·Biochimica Et Biophysica Acta·A Rodríguez-Navarro
Nov 15, 2001·Plant, Cell & Environment·N Moseyko, L J Feldman
Dec 1, 2001·Plant & Cell Physiology·A UedaT Takabe
Aug 1, 2002·The Plant Journal : for Cell and Molecular Biology·Wolf-N FischerWolf B Frommer
Jun 14, 2003·Plant Physiology·Quan-Sheng QiuKaren S Schumaker
Dec 31, 2003·The Journal of Biological Chemistry·Gabriel SchaafNicolaus von Wirén
Oct 7, 2004·Plant Physiology·Julie E Hayes, Robert J Reid
Mar 18, 2006·Journal of Plant Physiology·Małgorzata Janicka-Russak, Grazyna Kłobus
Apr 8, 2006·Plant Methods·Alexander SchulteChristoph Plieth
May 9, 2006·Current Biology : CB·Yaodong YangErik Nielsen
Nov 29, 2008·Journal of Plant Physiology·Xiong-Yan ChenJae-Yean Kim
Dec 2, 2009·Physiologia Plantarum·Barbara J Hawkins, Samantha Robbins
Dec 28, 2011·Journal of Experimental Botany·Xiangqiang KongWeijiang Li
Dec 7, 2013·Journal of Experimental Botany·Lars H Wegner
Jan 7, 2014·Frontiers in Plant Science·Alexandre MartinièreNadine Paris
Sep 11, 2014·Journal of Experimental Botany·John A Raven, Martina A Doblin
Sep 1, 2015·Journal of Plant Physiology·D T Britto, H J Kronzucker

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Citations

Oct 29, 2020·The New Phytologist·María Nieves Aranda SiciliaKees Venema
May 26, 2021·Journal of Plant Physiology·Dev T BrittoHerbert J Kronzucker
Jun 29, 2021·Frontiers in Plant Science·Maike Cosse, Thorsten Seidel

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