PMID: 16232643Oct 20, 2005Paper

Biochemical preparation of L-ribose and L-arabinose from ribitol: a new approach

Journal of Bioscience and Bioengineering
Z AhmedK Izumori

Abstract

L-ribose and L-arabinose were prepared biochemically from ribitol via a two-step reaction, by which the complete oxidation of ribitol to L-ribulose (approximately 98%) was achieved by the reaction of washed cells of Acetobacter aceti IFO 3281. The produced L-ribulose was then used as a substrate for the production of L-ribose and L-arabinose. The isomerization of L-ribulose to L-ribose and L-arabinose was carried out using L-ribose isomerase (L-RI) of Acinetobacter sp. strain DL-28 and L-arabinose isomerase (L-AI) of Mycobacterium smegmatis, respectively. At equilibrium, the ratio of L-ribose: L-ribulose was 70:30 and that of L-arabinose: L-ribulose was 90: 10. After a simple purification treatment, both pentoses could be crystallized without the use of column chromatography. The crystals were confirmed as L-ribose and L-arabinose by High-performance liquid chromatography (HPLC), Infrared (IR), Nuclear magnetic resonance (NMR) and optical rotation measurements.

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Citations

Jan 20, 2009·Applied Microbiology and Biotechnology·M HelantoA Nyyssölä
Aug 30, 2011·Applied Microbiology and Biotechnology·Chao HuCuiying Hu
Aug 29, 2009·Bioprocess and Biosystems Engineering·Young-Ju JeonIn-Ho Kim
Oct 20, 2005·Journal of Bioscience and Bioengineering·R M MizanurK Izumori
Dec 12, 2007·Bioscience, Biotechnology, and Biochemistry·G TakataK Izumori
Jul 11, 2007·Applied Microbiology and Biotechnology·Cassandra De MuynckErick Vandamme
Oct 5, 2014·Applied Microbiology and Biotechnology·Zheng XuHong Xu
Dec 5, 2012·Journal of Bioscience and Bioengineering·Kenji MorimotoK Izumori
Nov 13, 2008·Journal of Bioscience and Bioengineering·Yoshikiyo Sakakibara, Badal C Saha
Jun 9, 2004·Chembiochem : a European Journal of Chemical Biology·Joachim Jose, Steffen von Schwichow
Jan 4, 2019·Angewandte Chemie·Litavadee ChuaboonPimchai Chaiyen
Sep 18, 2007·Applied and Environmental Microbiology·M HelantoA Nyyssölä
Dec 1, 2007·International Journal of Systematic and Evolutionary Microbiology·Eun-Ah ChoYu-Ryang Pyun
Jun 12, 2021·Food Research International·Shahid MahmoodWanmeng Mu

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