PMID: 8988237Dec 16, 1996Paper

Biochemical properties of acetyl- and butyrylcholinesterase in human meningioma

Biochimica Et Biophysica Acta
J Sáez-Valero, C J Vidal

Abstract

The structural properties of acetyl-(AChE) and butyrylcholinesterase (BuChE) in meningioma and the possible relationship with brain and plasma were investigated. Meningioma ChEs were extracted with saline and saline-Triton X-100 buffers. The tumor ChE forms were identified by sedimentation analysis, and their amphiphilic/hydrophilic behaviour was assessed by Triton X-114 phase-partitioning and hydrophobic chromatography. Meningioma contained amphiphilic globular AChE dimers (G2A) and monomers (G1A), and hydrophilic BuChE tetramers (G4H). The conversion of G2A into G1A AChE by reduction confirmed their structures. In contrast to the meningioma species, brain G1A AChE forms remained amphiphilic after incubation with alkaline hydroxylamine and phosphatidylinositol-specific phospholipase C (PIPLC). Meningioma G1A and PIPLC-converted G1H, and brain G1A AChE showed similar rate constants for thermal inactivation, and this suggested that the thermal stability of AChE subunits was unaffected by the presence or not of phosphatidylinositol residues. AChE in meningioma and brain did not differ in the interaction with the lectins Con A, LCA, WGA and RCA. BuChE in meningioma and brain bound to a similar extent to Con A, LCA and WGA-Agarose,...Continue Reading

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Citations

May 18, 1999·Neuroscience Letters·M S García-AyllónC J Vidal
Apr 4, 2003·The International Journal of Biochemistry & Cell Biology·José Luis GómezCecilio J Vidal
May 10, 2002·Breast Cancer Research and Treatment·Francisco Ruiz-EspejoCecilio J Vidal
Aug 2, 2003·Breast Cancer Research and Treatment·Francisco Ruiz-EspejoCecilio J Vidal
Oct 5, 2010·The FEBS Journal·Encarnación Muñoz-DelgadoCecilio J Vidal
Nov 1, 2005·Chemico-biological Interactions·Cecilio J Vidal
Jan 23, 2008·Biological Chemistry·María F MontenegroCecilio J Vidal
Sep 29, 2005·Journal of Basic and Clinical Physiology and Pharmacology·Kunga Mohan RamkumarRajendran Ananthan
May 8, 2001·Medical and Pediatric Oncology·J P Perentesis

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