Biochemical properties of Paracoccus denitrificans FnrP: reactions with molecular oxygen and nitric oxide

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Jason C CrackNick E Le Brun

Abstract

In Paracoccus denitrificans, three CRP/FNR family regulatory proteins, NarR, NnrR and FnrP, control the switch between aerobic and anaerobic (denitrification) respiration. FnrP is a [4Fe-4S] cluster-containing homologue of the archetypal O2 sensor FNR from E. coli and accordingly regulates genes encoding aerobic and anaerobic respiratory enzymes in response to O2, and also NO, availability. Here we show that FnrP undergoes O2-driven [4Fe-4S] to [2Fe-2S] cluster conversion that involves up to 2 O2 per cluster, with significant oxidation of released cluster sulfide to sulfane observed at higher O2 concentrations. The rate of the cluster reaction was found to be ~sixfold lower than that of E. coli FNR, suggesting that FnrP can remain transcriptionally active under microaerobic conditions. This is consistent with a role for FnrP in activating expression of the high O2 affinity cytochrome c oxidase under microaerobic conditions. Cluster conversion resulted in dissociation of the transcriptionally active FnrP dimer into monomers. Therefore, along with E. coli FNR, FnrP belongs to the subset of FNR proteins in which cluster type is correlated with association state. Interestingly, two key charged residues, Arg140 and Asp154, that have...Continue Reading

References

Jan 1, 1991·Journal of Bacteriology·P J Kiley, W S Reznikoff
Jan 1, 1988·Journal of Bacteriology·D RaltS R Tannenbaum
May 7, 1985·Biochemistry·C T PrzysieckiM A Cusanovich
May 15, 1983·Journal of Molecular Biology·D J ShawJ R Guest
Mar 1, 1997·Methods : a Companion to Methods in Enzymology·J C Drapier
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·C V PopescuP J Kiley
May 13, 1999·Biochimica Et Biophysica Acta·N J WatmoughS Spiro
May 14, 1999·Current Opinion in Microbiology·G Sawers
Aug 12, 1999·Cellular and Molecular Life Sciences : CMLS·M C Broillet
Oct 6, 2000·Microbiology·M I Hutchings, S Spiro
Apr 12, 2001·European Journal of Biochemistry·M F OttenR J Van Spanning
Jun 16, 2001·Advances in Microbial Physiology·J GreenJ R Guest
Oct 3, 2001·The Journal of Biological Chemistry·L J Moore, P J Kiley
Dec 3, 2003·The Journal of Biological Chemistry·Jason CrackAndrew J Thomson
Jan 21, 2004·Biochemistry·Victoria R SuttonPatricia J Kiley
Feb 22, 2005·Molecular Aspects of Medicine·Richard Bruckdorfer
Jan 19, 2006·Biochemical Society Transactions·R VeldmanR J M van Spanning
May 24, 2006·The Journal of Biological Chemistry·Jason C CrackAndrew J Thomson
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jason C CrackAndrew J Thomson
Oct 13, 2007·BMC Bioinformatics·Daniel R CaffreyEnoch S Huang
Jan 12, 2008·Journal of the American Chemical Society·Jason C CrackAndrew J Thomson
Mar 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Adrian J JervisJeffrey Green
Jun 30, 2009·Journal of Bacteriology·Young-Man Kwon, Bernard Weiss
Nov 7, 2009·Methods in Enzymology·Aixin Yan, Patricia J Kiley
Nov 18, 2009·The Journal of Biological Chemistry·James EdwardsJames W B Moir
Dec 25, 2010·Journal of the American Chemical Society·Jason C CrackNick E Le Brun
Feb 5, 2011·Current Opinion in Chemical Biology·Angela S Fleischhacker, Patricia J Kiley
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Mar 28, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lars R BakkenAsa Frostegård
Sep 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bo ZhangMichael K Johnson
Mar 9, 2013·The Journal of Biological Chemistry·Jason C CrackNick E Le Brun
Mar 19, 2014·Methods in Molecular Biology·Jason C CrackNick E Le Brun

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Citations

Oct 3, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Dec 9, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Oct 23, 2019·International Journal of Molecular Sciences·Pierre AlbinaBenjamin Erable
Jun 14, 2018·Frontiers in Microbiology·Alfonso Olaya-AbrilMaría D Roldán
Dec 23, 2017·FEMS Microbiology Letters·Hannah GaimsterGary Rowley
May 25, 2021·Frontiers in Molecular Biosciences·Sylvain Durand, Maude Guillier

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Methods Mentioned

BETA
gel filtration
circular dichroism

Software Mentioned

Clustal Omega
Origin
Genedoc

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