PMID: 22587781May 17, 2012Paper

Biochemical property and in vivo efficacies of novel Val/Arg-rich antimicrobial peptide

Protein and Peptide Letters
Qingquan MaWen-Yu Sun

Abstract

A novel α-helical antimicrobial peptide G6 rich in Val/Arg residues has been screened previously. In this study, we further evaluated the biochemical stability, interaction with whole bacteria, and in vivo therapeutic or prophylactic role of the peptide in Salmonella typhimurium-infected mice. The results showed that G6 exhibited strong resistance to pH, heat, and salts. But G6 lost the antimicrobial activity when treated with proteolytic enzymes. G6 had no toxicity on mammalian cell. An intraperitoneal model of sepsis caused by Salmonella typhimurium was established in mice. G6 was administered intraperitoneally 1 h before or after mice were infected with Salmonella typhimurium. For the mice given peptide post-bacterial infection, the mortality of the mice and the peritoneal bacterial counts were significantly lower in the groups that were administered 2.5 mg/kg BW and 5.0 mg/kg BW of G6 (P < 0.05) compared to the PBS-treated group. Similar trend was obtained when G6 was given 1 h prior to Salmonella typhimurium infection. Peptide-membrane experiments showed that G6 was effective in permeabilizing the outer and inner membrane in a dose dependent manner, indicating that the peptide targets the cell membrane. Taken together, the...Continue Reading

Citations

Nov 26, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Tonghui YiYuxin Chen
Mar 22, 2014·Chemical Biology & Drug Design·Qingquan MaAnshan Shan
Oct 26, 2018·Medicinal Research Reviews·Jiajun WangAnshan Shan
Nov 5, 2016·Chemical Biology & Drug Design·Xiting LvAnshan Shan

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