Biochemical quantification of total brain glycogen concentration in rats under different glycemic states

Neurochemistry International
Florence MorgenthalerRolf Gruetter

Abstract

All (13)C NMR studies of brain glycogen to date relied on observing the incorporation of (13)C label into glycogen, and thus interpretation was potentially affected by changes in (13)C label turnover rates. The goal of this study was to quantify total brain glycogen concentration under conditions of hypoglycemia or normoglycemia using biochemical methods. Rats were sacrificed using a focused microwave fixation device. The results showed that metabolism of brain glycogen was Glc- and insulin-sensitive and that insulin-induced hypoglycemia promoted a gradual glycogenolysis. Moreover, we show that there are very mild effects of isoflurane and alpha-chloralose anesthesia on brain glycogen concentration. Altogether these results show that total brain glycogen serves as a substantial source of glucosyl units during insulin-induced moderate hypoglycemia and therefore may be neuroprotective. Finally we also conclude that previous interpretation of (13)C NMR spectroscopy data accurately reflected the changes in total brain glycogen content.

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Citations

Jan 12, 2008·Endocrinology·Raimund I HerzogRobert S Sherwin
May 12, 2009·Neurochemistry International·Florence D MorgenthalerRolf Gruetter
Oct 9, 2007·Neurobiology of Disease·Peter A AbdelmalikPeter L Carlen
Apr 27, 2007·Neurobiology of Disease·Peter A AbdelmalikPeter L Carlen
Nov 18, 2008·Journal of Neurochemistry·Florence D MorgenthalerRolf Gruetter
Jan 10, 2012·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Ruud B van HeeswijkRolf Gruetter
Jan 25, 2011·Journal of Neuroscience Research·Sarah E CanadaBartholomew A Pederson
Apr 4, 2015·Chinese Medical Journal·Li-Yun Yu, Yu Pei
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Oct 6, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gülin ÖzElizabeth R Seaquist
Dec 15, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Cristina CudalbuRolf Gruetter
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Nov 3, 2016·Journal of Diabetes Science and Technology·Lykke Blaabjerg, Claus B Juhl
Sep 26, 2020·Frontiers in Cellular Neuroscience·Rebecca StevensonYossi Buskila
Dec 31, 2016·Analytical Biochemistry·Ana Francisca SoaresHongxia Lei
Aug 10, 2012·The Journal of Experimental Biology·Jorge J Pérez-MaceiraManuel Aldegunde

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