Biochemical reconstitution defines new functions for membrane-bound glycosidases in assembly of the bacterial cell wall.

Proceedings of the National Academy of Sciences of the United States of America
Atsushi TaguchiSuzanne Walker

Abstract

The peptidoglycan cell wall is a macromolecular structure that encases bacteria and is essential for their survival. Proper assembly of the cell wall requires peptidoglycan synthases as well as membrane-bound cleavage enzymes that control where new peptidoglycan is made and inserted. Previous studies have shown that two membrane-bound proteins in Streptococcus pneumoniae, here named MpgA and MpgB, are important in maintaining cell wall integrity. MpgA was predicted to be a lytic transglycosylase based on its homology to Escherichia coli MltG, while the enzymatic activity of MpgB was unclear. Using nascent peptidoglycan substrates synthesized in vitro from the peptidoglycan precursor Lipid II, we report that both MpgA and MpgB are muramidases. We show that replacing a single amino acid in E. coli MltG with the corresponding amino acid from MpgA results in muramidase activity, allowing us to predict from the presence of this amino acid that other putative lytic transglycosylases actually function as muramidases. Strikingly, we report that MpgA and MpgB cut nascent peptidoglycan at different positions along the sugar backbone relative to the reducing end, with MpgA producing much longer peptidoglycan oligomers. We show that the cl...Continue Reading

References

Oct 27, 2001·Applied and Environmental Microbiology·C K SungD A Morrison
May 21, 1962·Biochimica Et Biophysica Acta·D CARLSTROM
Oct 5, 2007·Journal of the American Chemical Society·Deborah L PerlsteinSuzanne Walker
Feb 13, 2008·FEMS Microbiology Reviews·Waldemar VollmerSimon Foster
Apr 24, 2008·Molecular Microbiology·Girbe BuistOscar P Kuipers
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hak Suk ChungDaniel Kahne
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Thomas J SilhavySuzanne Walker
May 17, 2011·Journal of the American Chemical Society·Tsung-Shing Andrew WangSuzanne Walker
Dec 24, 2011·Analytical Biochemistry·Nhat Khai BuiWaldemar Vollmer
Jul 3, 2013·Cold Spring Harbor Perspectives in Medicine·Kelly S DoranMarc Lecuit
Jul 16, 2013·Environmental Microbiology·Orietta MassiddaWaldemar Vollmer
Jul 1, 2014·Nature Communications·Stéphane MesnageMichael P Williamson
Dec 17, 2014·Scientific Reports·Daniel KühnerUte Bertsche
Apr 8, 2015·Antimicrobial Agents and Chemotherapy·Ozden KocaogluErin E Carlson
Oct 29, 2015·Molecular Microbiology·Rachel YunckThomas G Bernhardt
Jun 23, 2016·Cold Spring Harbor Perspectives in Medicine·Karen Bush, Patricia A Bradford
Aug 16, 2016·Nature·Alexander J MeeskeDavid Z Rudner
Dec 13, 2016·Nature Microbiology·Andrew K FentonThomas G Bernhardt
May 30, 2017·Nature Chemical Biology·Yuan QiaoDaniel Kahne
Jun 24, 2017·Critical Reviews in Biochemistry and Molecular Biology·David A DikShahriar Mobashery
Jul 16, 2017·Molecular Microbiology·Gro Anita StamsåsLeiv Sigve Håvarstein
Nov 29, 2017·Journal of the American Chemical Society·Michael A WelshSuzanne Walker
Feb 7, 2018·Journal of the American Chemical Society·Kaitlin SchaeferSuzanne Walker
Mar 2, 2018·Annual Review of Biochemistry·Cathleen Zeymer, Donald Hilvert
Dec 6, 2020·Molecular Microbiology·Jessica L BohrhunterThomas G Bernhardt

❮ Previous
Next ❯

Citations

Sep 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·Joshua D Shirley, Erin E Carlson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.