PMID: 6985268Oct 1, 1982Paper

Biochemical separation of a human B cell mitogenic factor

Proceedings of the National Academy of Sciences of the United States of America
A MaizelR J Ford

Abstract

Recent studies have established the ability of human B lymphocytes to undergo G1-phase cell cycle progression and subsequent DNA synthesis upon exposure to factor(s) present in media conditioned by lectin-stimulated mononuclear cells. Procedures for the isolation of such a cytokine have been the focus of the present investigation. Conditioned medium from cells stimulated by lectin for 72 hr was fractionated by ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration chromatography. During the isolation procedure the proliferation-stimulating activity of the column fractions was assayed concurrently on purified human T cells, purified human B cells, and murine thymocytes. T cell and B cell stimulatory factors present in the initial conditioned medium were found to copurify during ammonium sulfate precipitation, DEAE-Sephadex chromatography, and Bio-Gel P-30 gel filtration. However, partial separation of these two activities was achieved after Bio-Gel P-100 gel filtration. Analytic polyacrylamide gel electrophoresis of radiolabeled Bio-Gel P-100 column fractions demonstrated a distinct protein band of 14,000-15,000 daltons in those column fractions predominantly supporting T cell growth and a distinct prote...Continue Reading

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Citations

Jul 1, 1987·Journal of Clinical Immunology·T MiyawakiM D Cooper
Sep 1, 1984·Journal of Clinical Immunology·A MuraguchiA S Fauci
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