Abstract
Type 1 Diabetes Mellitus (T1DM) is the autoimmune disorder of destruction of β cells of pancreas, creating insulin deficiency condition, which leads to hyperglycemia, polyuria, polydipsia, ketoacidosis, and other metabolic disorder especially in children. Different genetic aspects and environmental factors are involved in pathophysiology of the disease. About 20 genes are associated with this disease in which the most common is the different combination of haplotype DRB1-DQA1-DQB1 present at HLA gene. At HLA-DQB1, there are some SNPs which are associated with T1DM. In T1DM, there are number of biochemical, serological parameters which show some abnormalities leading to some complications. Samples were subjected to all biochemical and serological techniques to get the measurement of concentration of glucose, lipid profile (cholesterol, triglycerides, and HDL and LDL cholesterol), urea, creatinine, albumin, insulin, anti-insulin antibodies, C-peptides, and leptin. All these values were compared with controls values and statistical analysis was also done on these values. At molecular level, two primers set which were allele specific at HLA-DQB1, were used to amplify the SNPs, homozygous and heterozygous conditions were stated. PCR...Continue Reading
References
Jul 13, 2007·Diabetes Care·Richard M WatanabeThomas A Buchanan
Feb 7, 2008·Diabetes·Henry ErlichUNKNOWN Type 1 Diabetes Genetics Consortium
May 17, 2008·Trends in Molecular Medicine·Houria Ounissi-Benkalha, Constantin Polychronakos
Jun 13, 2008·Pediatric Diabetes·T SegalJ Allgrove
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Daniel KrausBarbara B Kahn
Sep 16, 2010·Acta Diabetologica·S B SahinC Yilmaz
Feb 19, 2013·Diabetic Medicine : a Journal of the British Diabetic Association·A G Jones, A T Hattersley
Dec 21, 2013·Diabetes Care·UNKNOWN American Diabetes Association
Dec 26, 2013·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·G IacobellisR Goldberg
Apr 22, 2015·JAMA : the Journal of the American Medical Association·Ezio BonifacioUNKNOWN Pre-POINT Study Group
Feb 26, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ramesh RamGrant Morahan
Jun 16, 2016·Lancet·Flemming Pociot, Åke Lernmark
Mar 31, 2017·Nature Reviews. Disease Primers·Anastasia KatsarouÅke Lernmark
Apr 30, 2017·Genome Biology·Vasiliki MatzarakiAlexandra Zhernakova
Nov 29, 2017·PloS One·Anna ParkkolaUNKNOWN Finnish Pediatric Diabetes Register
Jun 19, 2018·Molecular and Cellular Endocrinology·Denis M NyagaJustin M O'Sullivan