PMID: 3089299Aug 6, 1986Paper

Biochemical studies on a 12-lipoxygenase in human uterine cervix

Biochimica Et Biophysica Acta
S FlatmanT F Slater

Abstract

Human uterine cervix possesses a high 12-lipoxygenase activity; this enzyme has been isolated in a purified form from the squamous epithelial region of human cervix and its major properties have been investigated. Enzyme activity was present in all subcellular fractions obtained by centrifugation; the highest specific activity was associated with the microsome fraction (160,000 X g pellet). Purification of the enzyme was achieved by acetone precipitation, ion exchange chromatography on CM-cellulose and affinity chromatography on linoleyl-aminoethyl-Sepharose. The product from the incubation of sodium [1-14C]arachidonate with crude enzyme extracts co-chromatographed with authentic 12-hydroxyeicosatetraenoic acid, but the purified enzyme gave a product that behaved like the 12-hydroperoxy derivative. The enzyme had optimum activity at pH 6.5, a Km of 15 microM for arachidonic acid and was stimulated by ATP and Ca2+. Enzyme activity was inhibited by esculetin, nordihydroguaiaretic acid, eicosatetraynoic acid, detergents at concentrations greater than 0.1% (w/v) and preincubation of substrate with GSH and GSH peroxidase. The occurrence of a high 12-lipoxygenase activity is discussed in relation to the specific physiological functio...Continue Reading

References

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Citations

Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·T Yoshimoto, S Yamamoto
May 1, 1988·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S FlatmanT F Slater
Apr 1, 1994·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K A Akhilender NaiduA P Kulkarni
Dec 1, 1987·Immunological Investigations·P Davies
Jan 1, 1990·Journal of Biochemical Toxicology·J Z Byczkowski, A P Kulkarni
Aug 14, 1999·International Journal of Cancer. Journal International Du Cancer·S NigamS Yamamoto
Mar 1, 1997·Progress in Lipid Research·S YamamotoN Ueda
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Emanuela Camera, Mauro Picardo

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