Biochemical studies on an acidophilic, thermophilic bacterium, Bacillus acidocaldarius: isolation of bacteria, intracellular pH, and stabilities of biopolymers

Journal of Biochemistry
T OshimaM Baba

Abstract

Acidophilic, thermophilic bacteria were isolated from Japanese acidic hot springs. They were spore-forming rods, identified as Bacillus acidocaldarius. DNA extracted from these acido-thermophiles showed no abnormality in chemical structure; it was instantly denatured and gradually decomposed giving rise to apurinic acid in a hot acid environment milder than the optimal conditions for the growth of the acido-thermophiles. Glyceraldehyde-3-phosphate dehydrogenase extracted from B. acidocaldarius was not active at pH 5 or less, and was resistant to heat at neutral but not acid pH. The intracellular pH was computed to be neutral by using dimethyl-2,4-oxazolidinedione. When uncouplers or inhibitors of respiration were added to the cells suspended in hot acid solution, the estimated pH was not changed and glyceraldehyde-3-phosphate dehydrogenase in the cells was not denatured. These results suggest that the cytoplasm of B. acidocaldarius is a hot neutral environment, and that a pH gradient across the cell envelope can be maintained even when oxidative phosphorylation or respiration is inhibited.

Citations

Apr 14, 2018·Indian Journal of Microbiology·Low Yi YikKenneth Francis Rodrigues
Sep 1, 1980·Journal of Bacteriology·H Kobayashi, T Unemoto
Dec 1, 1983·Microbiological Reviews·J G Cobley, J C Cox
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Dec 1, 1981·Biochimica Et Biophysica Acta·E PadanS Schuldiner
Mar 3, 2007·Trends in Microbiology·Craig Baker-Austin, Mark Dopson

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