Biochemical studies on pili isolated from Pseudomonas aeruginosa strain PAO

Canadian Journal of Microbiology
W ParanchychT H Watts


Pseudomonas aeruginosa strains PAO and PAK bear polar pili which are flexible filaments having a diameter of 6 nm and an average length of 2500 nm. Both types of pili are retractile and promote infection by a number of bacteriophages. The present communication describes the partial biochemical characterization of PAO pili isolated from a multipiliated nonretractile mutant of PAO. The observed properties are compared to those of PAK pili which were characterized previously. PAO pili were found to contain a single polypeptide subunit of 18 700 daltons. This is similar to PAK pili which contain a single polypeptide of 18 100 daltons. The amino acid composition of PAO pilin was also similar to that of PAK pilin. Neither protein contained phosphate or carbohydrate residues and both were found to contain N-methylphenylalanine at the amino terminus. Sequencing of 20 amino acid residues at the amino terminal end of PAO pilin revealed the sequence to be identical with that of PAK pilin, while tryptic peptide analyses of PAO and PAK pilin indicated that the two proteins probably contain a number of homologous regions within the polypeptide. It was concluded that PAO and PAK pili were closely related structures.


Mar 1, 1989·Molecular & General Genetics : MGG·P CastricJ C Sadoff
May 16, 2002·Infection and Immunity·Jason E ComerP Castric
Feb 3, 2005·Journal of Bacteriology·Erin J van SchaikR T Irvin
Jul 4, 2012·Annual Review of Microbiology·Lori L Burrows
Mar 21, 2015·FEMS Microbiology Reviews·Jamie-Lee Berry, Vladimir Pelicic
Jul 19, 1983·Biochemistry·T H WattsW Paranchych
Jan 24, 1983·FEBS Letters·P A SastryW Paranchych
Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Sandy Y M NgKen F Jarrell
Nov 1, 1987·Molecular Microbiology·T C Elleman, J E Peterson
Jun 1, 1989·FEMS Microbiology Letters·M SatoT Miwatani
Jan 1, 1983·Critical Reviews in Microbiology·G W Jones, R E Isaacson
Apr 11, 2001·The Journal of Biological Chemistry·D W KeizerB D Sykes
May 9, 2001·The Journal of Biological Chemistry·P CastricR W Carlson
Aug 18, 2004·Journal of Medical Microbiology·A P FonsecaJ C Sousa
Dec 1, 1998·The Journal of Peptide Research : Official Journal of the American Peptide Society·P J CachiaR S Hodges
Oct 12, 1999·The American Journal of Physiology·W I MariencheckJ R Wright
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M S ReddyW Paranchych

Related Concepts

Bacterial Proteins
Pseudomonas aeruginosa
Cutis Gyrata Syndrome of Beare And Stevenson
Bacterial Pilus

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.