Biochemistry of vibrio cholerae virulence. II. Skin permeability factor-cholera enterotoxin production in a chemically defined medium.

Infection and Immunity
L T CallahanS H Richardson

Abstract

A chemically defined medium capable of eliciting high titers of skin permeability factor (PF)/enterotoxin from three different strains of Vibrio cholerae has been developed. Toxin/antigen elaboration in synthetic and in complex media was monitored by a specific passive hemagglutination-inhibition test. A distinct temporal difference in the pattern of toxin/antigen elaboration was noted when the two types of media were compared. In complex media, PF activity and corresponding antigen release were coincident, whereas in the defined medium a biphasic pattern resulting in elaboration of nontoxic antigen during the second phase was seen. Possible reasons for the latter observation are discussed, and several experiments illustrating the unique utility of the defined medium are presented.

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Citations

Sep 1, 1993·Protein Science : a Publication of the Protein Society·C J Jeffery, D E Koshland
Dec 7, 2011·Infection and Immunity·Alexandra R MeyShelley M Payne
Jul 1, 1990·Applied Microbiology and Biotechnology·M van de WalleJ Shiloach
Feb 20, 2007·The Journal of Biological Chemistry·Jason T PrattAndrew Camilli
Jul 28, 2010·Molecular Microbiology·Jason T PrattAndrew Camilli
Jul 17, 2004·Molecular Microbiology·Anna D Tischler, Andrew Camilli
Jun 1, 1977·Infection and Immunity·J J MekalanosW R Romig
Oct 1, 1993·Applied and Environmental Microbiology·N Amirmozafari, D C Robertson
Mar 1, 1980·Infection and Immunity·B E Ivins, R K Holmes
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Aug 23, 2005·Infection and Immunity·Anna D Tischler, Andrew Camilli
Mar 1, 1977·Journal of Ultrastructure Research·P B FernandesH L Smith

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