Biochip functionalization using electrowetting-on-dielectric digital microfluidics for surface plasmon resonance imaging detection of DNA hybridization

Biosensors & Bioelectronics
Lidija MalicMaryam Tabrizian

Abstract

This work reports on a dynamically configurable micro-array surface plasmon resonance biochip platform. The platform comprises a digital electrowetting-on-dielectric (EWOD) microfluidic device tailored to surface plasmon resonance imaging (SPRi). We demonstrate its application for simultaneous immobilization of different DNA probes at the designated detection sites on-chip from sub-microL volume solutions in combination with multichannel label-free real-time detection of subsequent hybridization reactions. Successful on-chip DNA probe dilution and immobilization is also demonstrated using SPRi hybridization detection. Furthermore, active control of the immobilized probe density and orientation is achieved under an applied potential using the electric interface of the EWOD device. For low probe densities, under negative applied potential, the DNA hybridization efficiency is enhanced compared to passive probe immobilization, yielding a two-fold SPR signal increase within only 8min of hybridization. EWOD microfluidic platform coupled with SPRi promises to dramatically increase the speed of detection and quantification of biomolecular interactions while reducing reagent consumption. The proposed system would enable the development ...Continue Reading

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Citations

Nov 9, 2010·Analytical and Bioanalytical Chemistry·Elizabeth M MillerAaron R Wheeler
Nov 29, 2012·Analytical and Bioanalytical Chemistry·Roberta D'Agata, Giuseppe Spoto
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Oct 19, 2010·Biosensors & Bioelectronics·Abdennour AbbasQuan Cheng
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Feb 15, 2016·Micromachines·Jie-Long HeShih-Kang Fan

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