Biochromatographic framework for analyzing magnesium chloride salt dependence on nor-NOHA binding to arginase enzyme

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Teddy BagnostC Andre

Abstract

Our group demonstrated recently that arginase I inhibition reduces endothelial dysfunction and blood pressure rising in spontaneously hypertensive rats [C. Demougeot, A. Prigent-Tessier, C. Marie, A. Berthelot, J. Hypertens. 23 (2005) 971; C. Demougeot, A. Prigent-Tessier, T. Bagnost, C. Andre, Y. Guillaume, M. Bouhaddi, C. Marie, A. Berthelot, Life Sci. 80 (2007) 1128]. This discovery opens interesting perspectives in the development of new drugs against hypertension. As well, in a previous paper [T. Bagnost, Y.C. Guillaume, M. Thomassin, J.F. Robert, A. Berthelot, A. Xicluna, C. Andre, J. Chromatogr. B: Analyt. Technol. Biomed. Life Sci. 856 (2007) 113], a novel biochromatographic column was developed in our laboratory for studying the binding of N(omega)-hydroxy-nor-l-arginine (nor-NOHA), an arginase inhibitor, with this enzyme. In this manuscript, using this novel biochromatographic concept, the effect of magnesium chloride on the nor-NOHA/arginase binding was analyzed for the first time. This study demonstrated that the salt ions interacted with arginase and played a great role in the nor-NOHA/arginase association. For a salt concentration (x) in the medium less than 3mM, the nor-NOHA/arginase binding decreased with x due ...Continue Reading

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Jan 16, 2007·Life Sciences·Céline DemougeotAlain Berthelot
Jun 26, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Teddy BagnostClaire André

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Citations

Feb 12, 2011·Journal of Pharmaceutical and Biomedical Analysis·Claire AndréYves Claude Guillaume
Sep 25, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Claire AndréYves Claude Guillaume

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