Biocide-Tolerant Listeria monocytogenes Isolates from German Food Production Plants Do Not Show Cross-Resistance to Clinically Relevant Antibiotics

Applied and Environmental Microbiology
A RoedelS Vincze

Abstract

Contamination of food during processing is recognized as a main transmission route of Listeria monocytogenes To prevent microbial contamination, biocides are widely applied as disinfectants in food processing plants. However, there are concerns about the development of antimicrobial resistance in foodborne pathogens due to widespread biocide usage. In our study, 93 L. monocytogenes isolates from German food production facilities were (i) tested for biocide and antibiotic susceptibility using broth microdilution assays, (ii) analyzed for links between reduced biocide susceptibility and antibiotic resistance, and (iii) characterized by whole-genome sequencing, including the detection of genes coding for biocide tolerance, antibiotic resistance, and other virulence factors. Fifteen L. monocytogenes isolates were tolerant to benzalkonium chloride (BAC), and genes conferring BAC tolerance were found in 13 of them. Antibiotic resistance was not associated with biocide tolerance. BAC-tolerant isolates were assigned to 6 multilocus sequence type (MLST) clonal complexes, and most of them harbored internalin A pseudogenes with premature stop codons or deletions (n = 9). Our study demonstrated a high genetic diversity among the investigat...Continue Reading

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Citations

Feb 6, 2020·Microorganisms·Matthias NollCarolin Gold
Jan 18, 2021·International Journal of Food Microbiology·Adrián Alvarez-MolinaAvelino Alvarez-Ordóñez
Mar 4, 2021·Food Microbiology·Sanelisiwe Thinasonke DuzeMrudula Patel

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Software Mentioned

Quast
Ridom SeqSphere +
BayesHammer
FastQC
BacMet
BLAST +
MikroWin
HMMER
ResFinder
SPSS

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