Biocompatibility and membrane strength of C3H10T1/2 cell-loaded alginate-based microcapsules

Cytotherapy
W-J ZhangT-T Tang

Abstract

C3H10T1/2 cells, from a mouse embryonic fibroblast cell line, were used to investigate the improvement of alginate-based microencapsulated cells for cellular therapy. Purified sodium alginate (PSA) and non-purified sodium alginate (SA) were used to prepare alginate-based microcapsules, and their biocompatibility and membrane strength were then compared for the purposes of analyzing the advantages of purifying SA. In addition, poly-l-lysine (PLL) was replaced by chitosan for alginate-chitosan microcapsule preparation. The process of optimization and chemical modification of alginate-chitosan microcapsules using polyethylene glycol was also reviewed. The results showed improved biocompatibility and membrane strength of PSA-based microcapsules. Under optimal conditions, mesenchymal stromal cell (MSC)-loaded alginate-chitosan microcapsules with good morphology could be obtained using PSA and chitosans of medium molecular weight (1.0-2.5 x 10(5)). A chitosan solution of 0.1% (w/v) and a reaction time of 7 min between alginate and chitosan were determined as optimal preparation parameters. It could be concluded that the chemical modification of alginate-based microcapsules can improve their biocompatibility.

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Citations

Feb 4, 2014·International Journal of Biological Macromolecules·Sunita NayakSubhas C Kundu
Apr 30, 2013·International Journal of Pharmaceutics·Cong LiuLongxiao Liu
Mar 15, 2013·International Journal of Nanomedicine·Guichen ZhouYanqiang Zhong
Dec 20, 2011·Journal of Healthcare Engineering·Wujie Zhang, Xiaoming He
Sep 13, 2015·Journal of Biomedical Materials Research. Part a·Guoshuang ZhengXiaojun Ma
Feb 14, 2012·International Journal of Pharmaceutics·Hongkwan ParkJung Hyun Kim
Mar 19, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wujie ZhangXiaoming He
Aug 28, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Jiani ZhengXiaojun Ma
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Nov 4, 2016·Journal of Applied Biomaterials & Functional Materials·Rebecca L MajewskiDavid C Markel

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