Biocompatibility evaluation of Jordanian Portland cement for potential future dental application

Journal of Conservative Dentistry : JCD
Abubaker S QutieshatHoma Darmani

Abstract

The demand for novel Portland cement (PC)-based formulations to be used in dental applications is ever increasing in viewing the foregoing knowledge on the favorable effects of these formulations on cellular proliferation and healing, leading to treatment success. This study investigated the effect of white and gray mineral trioxide aggregate (W-MTA and G-MTA) and white and gray Jordanian PC (W-PC and G-PC) in their raw state on the viability of Balb/C 3T3 fibroblasts using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials were prepared in the form of disks, with a diameter of 5 mm and a thickness of 2 mm. In the first experiment, Balb/C 3T3 fibroblasts were cultured with the material disks using culture plate inserts. In the second experiment, material elutes were added to cultured cells. The elutes were prepared by adding 2 ml serum-free media to 10 disks of each material and then incubated at 37°C for different time intervals. Material elutes were analyzed using ion chromatography for traces of calcium. The data were analyzed using analysis of variance followed by Dunnett test (α = 0.05) or Tukey test (α = 0.05). In response to material disks, G-PC had a proliferative effect on cells at ...Continue Reading

References

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Citations

Oct 14, 2020·Microscopy Research and Technique·Cleonice da Silveira TeixeiraLucas da Fonseca Roberti Garcia

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Methods Mentioned

BETA
ELISA

Software Mentioned

Minitab

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