Biocompatibility of a Conjugated Polymer Retinal Prosthesis in the Domestic Pig

Frontiers in Bioengineering and Biotechnology
José Fernando Maya-VetencourtFabio Benfenati

Abstract

The progressive degeneration of retinal photoreceptors is one of the most significant causes of blindness in humans. Conjugated polymers represent an attractive solution to the field of retinal prostheses, and a multi-layer fully organic prosthesis implanted subretinally in dystrophic Royal College of Surgeons (RCS) rats was able to rescue visual functions. As a step toward human translation, we report here the fabrication and in vivo testing of a similar device engineered to adapt to the human-like size of the eye of the domestic pig, an excellent animal paradigm to test therapeutic strategies for photoreceptors degeneration. The active conjugated polymers were layered onto two distinct passive substrates, namely electro-spun silk fibroin (ESF) and polyethylene terephthalate (PET). Naive pigs were implanted subretinally with the active device in one eye, while the contralateral eye was sham implanted with substrate only. Retinal morphology and functionality were assessed before and after surgery by means of in vivo optical coherence tomography and full-field electroretinogram (ff-ERG) analysis. After the sacrifice, the retina morphology and inflammatory markers were analyzed by immunohistochemistry of the excised retinas. Surp...Continue Reading

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Citations

May 14, 2021·Journal of Neural Engineering·Sai Siva KareLaxman Saggere
Jun 1, 2021·International Journal of Pharmaceutics·Suzana Gonçalves CarvalhoJanaína Cecília Oliveira Villanova
Jul 14, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shambhavi BagewadiAnuradha Subramanian

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Methods Mentioned

BETA
chips
confocal microscopy
scanning electron microscopy

Software Mentioned

GraphPad Prism
OriginPro
WinAverager
Fiji
Prism

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