Biocompatibility of biological material polylactic acid with stem cells from human exfoliated deciduous teeth

Biomedical Reports
Xi WangQiang Sun

Abstract

To investigate the biocompatibility of the biomaterial, polylactic acid (PLA) with stem cells from human exfoliated deciduous teeth (SHED) and its induction of mineralization as a type of scaffold material. To determine the impacts of biomaterial PLA on proliferation and mineralization of SHED, the expression of surface molecules of SHED isolated and cultured in vitro was detected by flow cytometry. In addition, cell proliferation was measured using MTT and Edu assays, and the evaluation of mineralized differentiation was performed using Alizarin Red S staining. In addition, the expression levels of osteogenic marker genes were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. SHED were successfully isolated and identified. The MTT and Edu results indicated that the proliferation of SHED cultured in PLA and normal medium was not significantly different. The Alizarin Red S staining demonstrated that the mineralization capability was significantly higher in the SHED that were cultured in PLA medium. Furthermore, RT-qPCR and western blot analyses indicated that the expression levels of osteogenic marker genes were higher in the SHED cultured in PLA medium. These results s...Continue Reading

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Citations

Jan 24, 2018·International Journal of Molecular Sciences·Francesca DiomedeOriana Trubiani
Oct 10, 2020·World Journal of Stem Cells·Cornelia Larissa Granz, Ali Gorji
Feb 25, 2020·Journal of the Mechanical Behavior of Biomedical Materials·Fahad AlamS Kumar

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Methods Mentioned

BETA
flow cytometry
PCR
protein assay

Software Mentioned

Image
SPSS
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