Biocompatibility of components of a female controlled drug delivery system

Journal of Biomedical Materials Research. Part a
Bharat K WarrierChi H Lee

Abstract

A female controlled drug delivery system (FcDDS) containing sodium dodecyl sulfate as a microbicide, ethylenediaminetetraacetic acid (EDTA) as a synergistic microbicide, and lactic acid as a pH modulator was developed as an intravaginal barrier device against sexually transmitted diseases. The host response of the vagina to the FcDDS was evaluated through biocompatibility tests including cell viability, estrogenicity, and cytotoxicity assays on HeLa cervical cells and NIH:Ovcar-3 ovarian cells. Gel electrophoresis and reverse transcriptase polymerase chain reaction assays on HeLa cervical cell lines were also performed to elucidate the effects of EDTA on the expression of particular proteins of interest. The results of the cell viability test showed no significant difference in viability of cells upon exposure to EDTA at concentrations less than 0.035% that was reported to exert spermicidal activity. EDTA at concentrations less than 0.035% did not cause any cytotoxicity. The results of reverse transcriptase polymerase chain reaction analysis revealed that EDTA induced the expression of a 67-kDa protein in HeLa cells, which was identified as elastin binding protein (a part of the elastin receptor complex). This work has demonstr...Continue Reading

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Dec 9, 2003·Journal of Biomedical Materials Research. Part a·Eun Sang ChoeChi H Lee

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Citations

Apr 23, 2011·International Journal of Pharmaceutics·Meredith R ClarkPatrick F Kiser
Apr 20, 2006·International Journal of Pharmaceutics·J das Neves, M F Bahia

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