Biocompatibility of human osteosarcoma cells to root end filling materials

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Tsui Hsien HuangChia Tze Kao

Abstract

Ideal root end filling materials should have good physical and chemical properties, and the most important is that the material should be biocompatible with periradicular tissue. The biocompatibility of three root end filling materials, mineral trioxide aggregate, calcium hydroxide-based cement, and eugenol-based cement, were investigated in vitro by culturing extracts of these materials with human osteogenic sarcoma cells (U2OS). Extracts of each of the materials were made after incubation of the materials for 1 day and 1 week with complete McCoy's medium. The extracts were serially diluted and then incubated with U2OS cells for 24 and 48 h. Cell survival rates were assessed by means of a viability assay for mitochondrial dehydrogenase activity. Differences in mean cell survival rates were statistically assessed using one-way ANOVA. Results showed that the survival rates of U2OS cells were largest with mineral trioxide aggregate, followed by calcium hydroxide-based cement and eugenol-based cement at 24- and 48-h exposures using the 1-day and 1-week extracts. The duration of root end filling material extraction time and treatment time showed variable influence on the survival rates. The results suggest that mineral trioxide agg...Continue Reading

References

Jan 1, 1992·Dental Materials : Official Publication of the Academy of Dental Materials·J C WatahaC T Hanks
Jun 1, 1991·Endodontics & Dental Traumatology·S Friedman
Nov 1, 1986·The Journal of the American Dental Association·W R Hume
Sep 1, 1980·International Endodontic Journal·L Tronstad, A Wennberg
Jul 1, 1995·Journal of Endodontics·M TorabinejadT R Pitt Ford
Jun 1, 1993·Journal of Endodontics·G R BruceR J Sydiskis
Oct 1, 1995·Journal of Endodontics·M TorabinejadJ D Kettering
Jun 1, 1996·European Journal of Oral Sciences·H Schweikl, G Schmalz
Jun 26, 1998·Journal of Endodontics·R M OsorioA L Shawley
Feb 18, 1999·Biomaterials·P J MitchellF McDonald
Mar 11, 2003·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Marco Antonio Hungaro DuarteSylvio de Campos Fraga
Dec 23, 2003·Journal of Endodontics·Marcela Fridland, Rafael Rosado

❮ Previous
Next ❯

Citations

Nov 14, 2012·Journal of Endodontics·Bin-Na LeeWon-Mann Oh
Dec 17, 2008·Journal of Endodontics·Ming-You ShieShinn-Jyh Ding
Apr 26, 2008·Journal of Endodontics·Tsui-Hsien HuangShinn-Jyh Ding
Jun 29, 2014·BMC Complementary and Alternative Medicine·Rosa Huang LiuYew-Min Tzeng
Sep 7, 2013·Restorative Dentistry & Endodontics·Payal SaxenaVilas Newaskar
Aug 24, 2011·Brazilian Dental Journal·Suely Aparecida CunhaDanyel Elias da Cruz Perez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.