Biocompatibility of Intensified Decellularized Equine Carotid Arteries in a Rat Subcutaneous Implantation Model and in a Human In Vitro Model

Tissue Engineering. Part a
Niklas JeinsenUlrike Böer

Abstract

Limited biocompatibility of decellularized scaffolds is an ongoing challenge in tissue engineering. We recently demonstrated that intensified detergent-based decellularization of equine carotid artery (dEACintens) removed residual cellular molecules from the scaffold more efficiently than a conventional decellularization (dEACcon), although this approach did not eliminate its immunogenicity entirely. CCN1 has been shown to improve biocompatibility of dEACcon in a sheep model. In this study, we tested the biocompatibility of dEACintens and dEACcon with or without CCN1 coating after subcutaneous implantation in rats for up to 12 weeks. Explants were assessed by conventional histopathology and immunostaining for infiltrating M2 macrophages. Moreover, human macrophages derived from monocytes (MDM) or THP-1 cells (THP-derived macrophages [TDM]) were seeded onto dEACcon and dEACintens, and activation was assessed either by cytokine expression or matrix metalloprotease 2 and 7 staining. dEACintens showed a significantly reduced inflammatory infiltration (52%; p < 0.0001), as well as an earlier and denser neovascularization (1.4-fold, p < 0.0001) independent of CCN1 coating, which, however, reduced fibrosis exclusively with dEACintens ...Continue Reading

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Citations

Dec 12, 2019·Journal of Tissue Engineering and Regenerative Medicine·Claudia BilodeauMartin Post
Jun 20, 2019·Frontiers in Cardiovascular Medicine·Aneesh K RamaswamyJustin S Weinbaum
Jan 30, 2021·Neural Regeneration Research·Crystal Y LiAdrien A Eshraghi
Oct 17, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Aleksandra L PredeinaVladimir V Vinogradov
Apr 20, 2021·Advanced Healthcare Materials·Chao Tao, Dong-An Wang

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Methods Mentioned

BETA
Assay
fluorescence microscopy
density gradient centrifugation
scraping
PCR

Software Mentioned

GraphPad Prism

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