Biocompatibility of various collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells

Clinical Oral Implants Research
Daniel RothamelJürgen Becker

Abstract

The aim of the present study was to evaluate the biocompatibility of differently cross-linked collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells. Four collagen membranes [BioGide (BG), BioMend (BM), Ossix (OS) and TutoDent (TD)] were tested. Cells plated on culture dishes (CD) served as positive controls. Six specimens of each membrane were incubated with (1) human PDL fibroblasts [2 x 10(4) cells] (n=24), and (2) human osteoblast-like cells (SaOs-2) [2 x 10(4) cells] (n=24) under standardized conditions. After 7 days, adherent cells were stained with hematoxylin and counted using a reflected light microscope and the cell density per square millimeter was calculated. Additionally, cell morphology was investigated using scanning electron microscopy (SEM). Cell counts were presented as means and standard deviations (cells/mm(2)) and analyzed for statistical difference using the Wilcoxon test: (1) CD (434+/-76)>BG (64+/-19)=OS (61+/-8)>TD (44+/-4)>BM (12+/-5); (2) CD (453+/-92)>BG (94+/-46)=TD (84+/-49)>OS (41+/-23)>BM (0). SEM examination revealed that PDL fibroblasts adherent on BG, TD and OS appeared spindle-shaped and flat, like cells on CD. SaOs-2 osteoblasts adherent on CD were star shape...Continue Reading

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Mar 24, 2006·Journal of Materials Science. Materials in Medicine·E D O'CearbhaillP E McHugh
Sep 10, 2011·Tissue Engineering. Part C, Methods·Timo SchmidtLothar Just
Feb 2, 2013·Clinical and Translational Medicine·Will W Minuth, Lucia Denk
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Jun 7, 2008·Journal of Periodontology·Yuval ZuberyArie Goldlust
Apr 13, 2010·Journal of Periodontology·Ronaldo Barcellos de SantanaThomas Van Dyke
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Jan 15, 2014·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Yahya AçilMatthias Gierloff
Aug 8, 2014·Head & Face Medicine·Michelle Pereira Costa Mundim SoaresDenildo de Magalhães
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May 10, 2005·Clinical Oral Implants Research·Daniel RothamelJürgen Becker
Sep 2, 2009·Clinical Oral Implants Research·Avital KozlovskyCarlos E Nemcovsky
Jun 6, 2009·Clinical Oral Implants Research·Patrick H WarnkeStephan T Becker
Feb 5, 2009·Clinical Oral Implants Research·Ronald E JungChristoph H F Hammerle
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Feb 5, 2009·Clinical Oral Implants Research·Ofer MosesCarlos E Nemcovsky
Aug 16, 2006·Clinical Oral Implants Research·Frank SchwarzJürgen Becker

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