Biocompatible electrochemiluminescent biosensor for choline based on enzyme/titanate nanotubes/chitosan composite modified electrode

Biosensors & Bioelectronics
Hong DaiGuonan Chen

Abstract

A new biocompatible ECL biosensor based on enzyme/titanate nanotubes/chitosan composite film was developed for the determination of analytes in biological samples. In the fabrication of the new ECL biosensor, biocompatible titanate nanotubes (TNTs) and a model enzyme, i.e., choline oxidase (ChOX), were immobilized on a chitosan modified glassy carbon electrode (GCE) via electrostatic adsorption and covalent interaction, respectively. By this ECL biosensor, choline was enzymatically oxidized to hydrogen peroxide and detected by a sensitive luminol ECL system. The use of TNTs not only provided a biocompatible microenvironment for the immobilized enzyme, which resulted in an excellent stability and long lifetime of the ECL biosensor, but also exhibited great enhancement towards luminol ECL and thus led to a significant improvement in sensitivity of ECL biosensor. Satisfactory results were obtained when employing this biosensor in assaying the total choline in milk samples. The work would provide a common platform to develop various sensitive, selective and biocompatible ECL biosensors based on using enzyme/TNTs/CHIT composite films.

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Citations

Jul 12, 2012·Accounts of Chemical Research·Yafeng WuSongqin Liu
Sep 16, 2010·Chemical Communications : Chem Comm·Yafeng WuSongqin Liu
Apr 27, 2011·Chemical Communications : Chem Comm·Qixin DengMingdeng Wei
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Oct 28, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Fadoua SallemNadine Millot
Feb 13, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xueling ShanZhidong Chen
Sep 13, 2020·Biosensors·Emiliano Martínez-PeriñánEncarnación Lorenzo
Jul 17, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xueling ShanZhidong Chen
Jul 28, 2020·Frontiers in Chemistry·Huimin NiuZhenyu Liao
Sep 21, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ushula M TefasheJanine Mauzeroll

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