Biocompatible ELR-Based Polyplexes Coated with MUC1 Specific Aptamers and Targeted for Breast Cancer Gene Therapy

Molecular Pharmaceutics
Maria J PiñaFrancisco J Arias

Abstract

The search for new and biocompatible materials with high potential for improvement is a challenge in gene delivery applications. A cell type specific vector made of elastin-like recombinamer (ELR) and aptamers has been specifically designed for the intracellular delivery of therapeutic material for breast cancer therapy. A lysine-enriched ELR was constructed and complexed with plasmid DNA to give positively charged and stable polyplexes. Physical characterization of these polyplexes showed a particle size of around 140 nm and a zeta potential of approximately +40 mV. The incorporation of MUC1-specific aptamers into the polyplexes resulted in a slight decrease in zeta potential but increased cell transfection specificity for MCF-7 breast cancer cells with respect to a MUC1-negative tumor line. After showing the transfection ability of this aptamer-ELR vector which is facilitated mainly by macropinocytosis uptake, we demonstrated its application for suicide gene therapy using a plasmid containing the gene of the toxin PAP-S. The strategy developed in this work about using ELR as polymeric vector and aptamers as supplier of specificity to deliver therapeutic material into MUC1-positive breast cancer cells shows promising potential...Continue Reading

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Dec 21, 1993·Biochimica Et Biophysica Acta·L BarbieriF Stirpe
Apr 22, 2000·Cancer Treatment Reviews·K L CheungJ F Robertson
Dec 7, 2000·Critical Reviews in Therapeutic Drug Carrier Systems·S BlauA Rubinstein
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Kirsten Nielsen, Rebecca S Boston
Sep 8, 2001·Journal of Mammary Gland Biology and Neoplasia·J M BurchellJ Taylor-Papadimitriou
Nov 13, 2001·International Journal of Radiation Oncology, Biology, Physics·M T VlachakiE Aguilar-Cordova
Jan 5, 2002·Breast Cancer Research and Treatment·M V CroceA Segal-Eiras
Jun 27, 2006·Cellular and Molecular Life Sciences : CMLS·F Stirpe, M G Battelli
Oct 10, 2006·The AAPS Journal·Robert J Kreitman
Apr 6, 2007·The AAPS Journal·Xiang GaoDexi Liu
Aug 3, 2007·International Journal of Oncology·Sekhar DuraisamyDonald Kufe
Sep 1, 2007·Pharmaceutical Research·Tze-Haw Howard ChenDarin Y Furgeson
Nov 13, 2007·Advances in Experimental Medicine and Biology·Ana Maria Gonzalez-AnguloGabriel N Hortobagyi
Jun 24, 2008·Trends in Biotechnology·Etgar Levy-NissenbaumOmid C Farokhzad
Dec 5, 2008·Chemical Reviews·Meredith A Mintzer, Eric E Simanek
Dec 24, 2008·Nucleic Acids Research·Cátia S M FerreiraJean Gariépy
May 19, 2009·International Journal of Pharmaceutics·Archana SwamiK C Gupta
Aug 4, 2009·Nuclear Medicine and Biology·Chiara Da PieveSotiris Missailidis
Oct 17, 2009·The AAPS Journal·Mohammed S Al-Dosari, Xiang Gao
Nov 17, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Paulo C BessaMargarida Casal
Nov 26, 2009·Nature Reviews. Cancer·Donald W Kufe
Mar 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gaurav SahayAlexander V Kabanov
Oct 26, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gayong ShimYu-Kyoung Oh
Jan 7, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Xiaoyang LingMichael Andreeff
Mar 15, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Biraja C DashAbhay Pandit
Mar 23, 2011·Molecular Pharmaceutics·Xingfang SuDarrell J Irvine

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Citations

Oct 21, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shufang DongJiying Yang
Aug 19, 2017·Current Medicinal Chemistry·Marina S SyrkinaMikhail A Rubtsov
Aug 2, 2017·Medicinal Research Reviews·Maryam Sadat NabaviniaKhalil Abnous
Jul 10, 2019·Matrix Biology : Journal of the International Society for Matrix Biology·Arturo Ibáñez-FonsecaJosé Carlos Rodríguez-Cabello
Dec 4, 2019·Cancer Letters·Maria J PiñaF Javier Arias
Nov 25, 2020·Pharmaceutics·Alessandra GirottiFrancisco Javier Arias
Oct 21, 2020·Advanced Drug Delivery Reviews·Laura ChambreDavid L Kaplan
Jul 8, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Reza MohammadinejadAli Zarrabi
Dec 23, 2016·Biochimica Et Biophysica Acta. General Subjects·Paula J BatesJohn O Trent
Apr 3, 2020·Molecular Pharmaceutics·Juan Gonzalez-ValdiviesoF Javier Arias
Apr 5, 2019·Biomacromolecules·Juan Gonzalez-ValdiviesoF Javier Arias

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