Biocompatible oil core nanocapsules as potential co-carriers of paclitaxel and fluorescent markers: preparation, characterization, and bioimaging

Colloid and Polymer Science
Sławomir Drozdek, Urszula Bazylińska

Abstract

The present work is focused on the long-term stability and in vitro cellular internalization of newly designed biocompatible polyester nanocapsules prepared via nanoprecipitation approach with mean diameter <165 nm and narrow size distribution, dedicated to theranostic applications. We monitored the optical, morphological, and biological properties of the nanocarriers loaded by multifunctional cargo, i.e., paclitaxel (PTX) and a fluorescent marker: coumarin-6 (CR-6) or Nile Red (NR), by fluorescence and UV-vis spectroscopy (encapsulation efficiency), dynamic light scattering (average size expressed as hydrodynamic diameter, D H), zeta potential (ζ, colloidal stability), atomic force microscopy (AFM, imaging), and confocal laser scanning microscopy (CLSM, nanocapsule visualization, and cellular internalization in vitro by human breast cancer MCF-7/WT cells). The fabricated nanocapsules with optimal composition of oleic phase, i.e., coconut oil, palm oil, and Capmul MCM, as well as polymeric shell, i.e., polylactic acid (PLA), poly (ε-caprolactone) (PCL), and poly (lactide-co-glycolide) (PLGA), showed high loading capacity, long-term stability, and improved localization of the active cargo in studied tumor cells. Therefore, our r...Continue Reading

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Citations

Feb 20, 2019·Biomolecules·Danial Efendy GoonMusalmah Mazlan
Sep 6, 2020·Polymers·Joanna Szafraniec-SzczęsnySzczepan Zapotoczny
Jan 28, 2021·International Journal of Molecular Sciences·Shira EngelbergYoav D Livney
Mar 31, 2021·International Journal of Pharmaceutics·Narimane LammariAbdelhamid Elaissari
May 28, 2021·International Journal of Nanomedicine·Dina FarinhaHenrique Faneca
Jul 1, 2021·Nanomedicine·Ahmed S AbdElhamidAhmed O Elzoghby
Feb 28, 2019·ACS Applied Materials & Interfaces·Joanna OdrobińskaSzczepan Zapotoczny

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Methods Mentioned

BETA
nanoprecipitation
dynamic light scattering
atomic force microscopy
fluorescence microscopy
AFM
imaging techniques

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