Biocomposite based on reduced graphene oxide film modified with phenothiazone and flavin adenine dinucleotide-dependent glucose dehydrogenase for glucose sensing and biofuel cell applications

Analytical Chemistry
Yehonatan RavennaLital Alfonta

Abstract

A novel composite material for the encapsulation of redox enzymes was prepared. Reduced graphene oxide film with adsorbed phenothiazone was used as a highly efficient composite for electron transfer between flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase and electrodes. Measured redox potential for glucose oxidation was lower than 0 V vs Ag/AgCl electrode. The fabricated biosensor showed high sensitivity of 42 mA M(-1) cm(-2), a linear range of glucose detection of 0.5-12 mM, and good reproducibility and stability as well as high selectivity for different interfering compounds. In a semibiofuel cell configuration, the hybrid film generated high power output of 345 μW cm(-2). These results demonstrate a promising potential for this composition in various bioelectronic applications.

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Citations

Dec 15, 2016·ACS Applied Materials & Interfaces·Jiang OuyangYou-Nian Liu
Jul 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazuya IshidaTakahide Kishimoto
Jul 6, 2016·Small·Dan Wen, Alexander Eychmüller
Jan 18, 2018·Nanotechnology Reviews·Hedieh MalekzadMichael R Hamblin
Oct 7, 2020·Chemical Society Reviews·Hazhir TeymourianJoseph Wang
Sep 3, 2020·Micromachines·Hye Suk HwangMincheol Chang
Sep 16, 2017·Journal of the American Chemical Society·Itay AlgovLital Alfonta

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