Biocontrol of Rhizoctonia solani Damping-Off of Tomato with Bacillus subtilis RB14.

Applied and Environmental Microbiology
O Asaka, Makoto Shoda

Abstract

Bacillus subtilis RB14, which showed antibiotic activities against several phytopathogens in vitro by producing the antibiotics iturin A and surfactin, was subjected to a pot test to investigate its ability to suppress damping-off of tomato seedlings caused by Rhizoctonia solani. To facilitate recovery from soil, B. subtilis RB14-C, a spontaneous streptomycin-resistant mutant of RB14, was used. Damping-off was suppressed when the culture broth, cell suspension, or cell-free culture broth of RB14-C was inoculated into soil. Iturin A and surfactin were recovered from the soils inoculated with the cell suspension of RB14-C, confirming that RB14-C produced them in soil. The gene lpa-14, which was cloned from RB14 and required for the production of both antibiotics, was mutated in RB14-C, and a mutant, R(Delta)1, was constructed. The level of disease suppressibility of R(Delta)1 was low, but R(Delta)1(pC115), a transformant of R(Delta)1 with the plasmid pC115 carrying lpa-14, was restored in suppressibility. These results show that the antibiotics iturin A and surfactin produced by RB14 play a major role in the suppression of damping-off caused by R. solani. RB14-C, R(Delta)1, and R(Delta)1(pC115) persisted in soil during the experi...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J LavilleD Haas
Jun 1, 1992·Applied and Environmental Microbiology·A R de la CruzM V Wiese
Oct 1, 1991·Applied and Environmental Microbiology·M N VincentE A Pierson
Sep 1, 1982·Applied and Environmental Microbiology·L N LiangM Alexander
Jun 1, 1994·Applied and Environmental Microbiology·L A Silo-SuhJ Handelsman

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Citations

Oct 29, 2000·Journal of Applied Microbiology·C ThraneJ Sørensen
Jun 6, 2007·Applied Microbiology and Biotechnology·Dequan LiZhiyi Chen
Oct 24, 2008·Phytopathology·Brian B McSpadden Gardener
Jul 14, 2010·Journal of Mass Spectrometry : JMS·Ylenia PecciGianna Allegrone
Mar 3, 2005·Applied Microbiology and Biotechnology·Marc OngenaPhilippe Thonart
Jun 12, 2012·Annual Review of Phytopathology·Jos M Raaijmakers, Mark Mazzola
Jan 15, 2015·World Journal of Microbiology & Biotechnology·María Emilia Rinland, Marisa Anahí Gómez
Feb 7, 2003·Applied and Environmental Microbiology·Tommy Harder Nielsen, Jan Sørensen
Apr 13, 2007·Molecular Plant-microbe Interactions : MPMI·Diego RomeroAlejandro Pérez-García
Oct 14, 2004·Journal of Applied Microbiology·P I KimY-T Chi
Apr 26, 2013·International Journal of Molecular Sciences·Xuliang ZhuangGuoqiang Zhuang
Oct 1, 2013·Biocontrol Science·Caroline F Ajilogba, Olubukola O Babalola
Jul 1, 2010·World Journal of Microbiology & Biotechnology·Sevdalina Todorova, Lubka Kozhuharova
Apr 1, 2009·Environmental Microbiology Reports·Venant NihorimbereMarc Ongena

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