Biocontrol potential of chitinases produced by newly isolated Chitinophaga sp. S167

World Journal of Microbiology & Biotechnology
Sonia SharmaRamandeep Kaur

Abstract

A chitinolytic bacterium Chitinophaga sp. S167 producing extracellular chitinases was isolated from a soil sample in India. The extracellular chitinases produced by S167 were concentrated by ammonium sulphate precipitation (AS70) and seven bands corresponding to chitinases were observed by zymography. Optimum temperature and pH of AS70 were between 40 and 45 °C and pH 6.0 respectively with high stability at 20-40 °C and pH 5-7. AS70 inhibited the growth of Fusarium oxysporum, Alternaria alternata and Cladosporium sp. in vitro. The culture conditions for the high level production of extracellular chitinases were optimized resulting in 48-folds higher chitinase production. As the combination of chitinases could be more potent in biocontrol of plant diseases, it was checked if AS70 could control postharvest fungal infection caused by Fusarium oxysporum on tomatoes. AS70 treated tomatoes showed significant lower incidence of infection (11%) by F. oxysporum as compared with 100% in the control at 5 days post inoculation. Further, AS70 caused significant mortality in second stage juveniles of root knot nematode, Meloidogyne incognita, a major agriculture pest responsible for economic losses in agriculture. This study highlights the a...Continue Reading

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Citations

Nov 12, 2020·Biology·Elisa Gamalero, Bernard R Glick
Nov 24, 2020·Archaea : an International Microbiological Journal·Abirami SasiDivya Venugopal
Apr 23, 2021·World Journal of Microbiology & Biotechnology·Zhenzhen ZhaoHai Yan

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Datasets Mentioned

BETA
KP017541
ACU59676.1

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Mega

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