Bioconversion of Cellulose to Acetate with Pure Cultures of Ruminococcus albus and a Hydrogen-Using Acetogen.

Applied and Environmental Microbiology
T L Miller, M J Wolin

Abstract

Bioconversion of cellulose to acetate was accomplished with cocultures of two organisms. One was the cellulolytic species Ruminococcus albus. It ferments crystalline cellulose (Avicel) to acetate, ethanol, CO(inf2), and H(inf2). The other organism (HA) obtains energy for growth by using H(inf2) to reduce CO(inf2) to acetate. HA is a gram-negative coccobacillus that was isolated from horse feces. Coculture of R. albus with HA in batch or continuous culture alters the fermentation products formed from crystalline cellulose by the ruminococcus via interspecies H(inf2) transfer. The major product of the fermentation by R. albus and HA coculture is acetate. High concentrations of acetate (333 mM) were obtained when batch cocultures grown on 5% cellulose were neutralized with Ca(OH)(inf2). Continuous cocultures grown at retention times of 2 and 3.1 days produced 109 and 102 mM acetate, respectively, when fed 1% cellulose with utilization of 84% of the substrate.

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Nov 1, 1988·Applied and Environmental Microbiology·S G PavlostathisM J Wolin
Nov 1, 1988·Applied and Environmental Microbiology·S G PavlostathisM J Wolin

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Citations

Apr 2, 2008·Annals of the New York Academy of Sciences·Harold L DrakeSteven L Daniel
Dec 20, 2018·Gut Microbes·Nick W SmithWarren C McNabb
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
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Mar 22, 2007·FEMS Microbiology Ecology·Kimberly L CookKinchel C Doerner
May 18, 2020·Applied Microbiology and Biotechnology·Yanfeng XueShengyong Mao
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May 21, 2019·Frontiers in Microbiology·Jingjing WangXingpeng Wang
Mar 7, 2017·Immune Network·Hiroko Nagao-Kitamoto, Nobuhiko Kamada

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