Bioconversion of dilute-acid pretreated sorghum bagasse to ethanol by Neurospora crassa

Applied Microbiology and Biotechnology
Ioannis DogarisDimitris Kekos

Abstract

Bioethanol production from sweet sorghum bagasse (SB), the lignocellulosic solid residue obtained after extraction of sugars from sorghum stalks, can further improve the energy yield of the crop. The aim of the present work was to evaluate a cost-efficient bioconversion of SB to ethanol at high solids loadings (16 % at pretreatment and 8 % at fermentation), low cellulase activities (1-7 FPU/g SB) and co-fermentation of hexoses and pentoses. The fungus Neurospora crassa DSM 1129 was used, which exhibits both depolymerase and co-fermentative ability, as well as mixed cultures with Saccharomyces cerevisiae 2541. A dilute-acid pretreatment (sulfuric acid 2 g/100 g SB; 210 °C; 10 min) was implemented, with high hemicellulose decomposition and low inhibitor formation. The bioconversion efficiency of N. crassa was superior to S. cerevisiae, while their mixed cultures had negative effect on ethanol production. Supplementing the in situ produced N. crassa cellulolytic system (1.0 FPU/g SB) with commercial cellulase and β-glucosidase mixture at low activity (6.0 FPU/g SB) increased ethanol production to 27.6 g/l or 84.7 % of theoretical yield (based on SB cellulose and hemicellulose sugar content). The combined dilute-acid pretreatment a...Continue Reading

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Citations

Mar 22, 2016·Bioresource Technology·Jorge A FerreiraMohammad J Taherzadeh
Apr 19, 2015·BMC Biotechnology·Thomas PaschosPaul Christakopoulos
Sep 19, 2015·Bioresource Technology·Manoj KoradiyaShailesh Dave
Sep 12, 2019·Biotechnology for Biofuels·Daria FeldmanOded Yarden
Apr 22, 2017·Journal of Industrial Microbiology & Biotechnology·Joshua C WatersKwangwon Lee

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