Bioconversion of hybrid poplar to ethanol and co-products using an organosolv fractionation process: optimization of process yields

Biotechnology and Bioengineering
Xuejun PanJack Saddler

Abstract

An organosolv process involving extraction with hot aqueous ethanol has been evaluated for bioconversion of hybrid poplar to ethanol. The process resulted in fractionation of poplar chips into a cellulose-rich solids fraction, an ethanol organosolv lignin (EOL) fraction, and a water-soluble fraction containing hemicellulosic sugars, sugar breakdown products, degraded lignin, and other components. The influence of four independent process variables (temperature, time, catalyst dose, and ethanol concentration) on product yields was analyzed over a broad range using a small composite design and response surface methodology. Center point conditions for the composite design (180 degrees C, 60 min, 1.25% H(2)SO(4), and 60% ethanol), yielded a solids fraction containing approximately 88% of the cellulose present in the untreated poplar. Approximately 82% of the total cellulose in the untreated poplar was recovered as monomeric glucose after hydrolysis of the solids fraction for 24 h using a low enzyme loading (20 filter paper units of cellulase/g cellulose); approximately 85% was recovered after 48 h hydrolysis. Total recovery of xylose (soluble and insoluble) was equivalent to approximately 72% of the xylose present in untreated wood...Continue Reading

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Citations

Feb 14, 2009·Applied Microbiology and Biotechnology·Xuebing ZhaoDehua Liu
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May 15, 2008·Applied Biochemistry and Biotechnology·Xuejun PanJack N Saddler
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Dec 1, 2015·Frontiers in Bioengineering and Biotechnology·Luis CaspetaJens Nielsen
Apr 19, 2008·Biotechnology and Bioengineering·Xuejun PanJack N Saddler
Jul 17, 2015·Bioprocess and Biosystems Engineering·Hamid Amiri, Keikhosro Karimi
Aug 13, 2014·Bioresource Technology·Maryam M KabirIlona Sárvári Horváth

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