Bioconversion of vitamin D to its active form by bacterial or mammalian cytochrome P450

Biochimica Et Biophysica Acta
Toshiyuki SakakiYoshitsugu Shiro

Abstract

Bioconversion processes, including specific hydroxylations, promise to be useful for practical applications because chemical syntheses often involve complex procedures. One of the successful applications of P450 reactions is the bioconversion of vitamin D₃ to 1α,25-dihydroxyvitamin D₃. Recently, a cytochrome P450 gene encoding a vitamin D hydroxylase from the CYP107 family was cloned from Pseudonocardia autotrophica and is now applied in the bioconversion process that produces 1α,25-dihydroxyvitamin D₃. In addition, the directed evolution study of CYP107 has significantly enhanced its activity. On the other hand, we found that Streptomyces griseolus CYP105A1 can convert vitamin D₃ to 1α,25-dihydroxyvitamin D₃. Site-directed mutagenesis of CYP105A1 based on its crystal structure dramatically enhanced its activity. To date, multiple vitamin D hydroxylases have been found in bacteria, fungi, and mammals, suggesting that vitamin D is a popular substrate of the enzymes belonging to the P450 superfamily. A combination of these cytochrome P450s would produce a large number of compounds from vitamin D and its analogs. Therefore, we believe that the bioconversion of vitamin D and its analogs is one of the most promising P450 reactions i...Continue Reading

References

Jun 1, 1990·Journal of Bacteriology·C A OmerD P O'Keefe
Jul 23, 1969·Biochemical and Biophysical Research Communications·M Horsting, H F DeLuca
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·E AxénK Wikvall
Jun 25, 1996·Biochemistry·M J BeckmanH F DeLuca
Apr 1, 1997·Steroids·S B Mahato, S Garai
Nov 5, 1997·Biochemical and Biophysical Research Communications·T MonkawaT Saruta
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·T ShinkiT Suda
Jul 13, 2000·Biochemical and Biophysical Research Communications·N SawadaK Inouye
Sep 30, 2000·European Journal of Biochemistry·T SakakiK Inouye
Oct 29, 2000·The Journal of Biological Chemistry·J J MüllerU Heinemann
Nov 2, 2001·Biochemical and Biophysical Research Communications·F HosseinpourK Wikvall
Jan 7, 2003·Nature Biotechnology·Florence Ménard SzczebaraBruno Dumas
Jul 16, 2003·Nature·Pamela A WilliamsHarren Jhoti
Jul 18, 2003·The Journal of Biological Chemistry·Jeffrey B ChengDavid W Russell
Mar 18, 2004·The Journal of Biological Chemistry·Tomoaki YamasakiYoshihiko Ohyama
Jun 23, 2004·Biochemical and Biophysical Research Communications·Natsumi SawadaKuniyo Inouye
Jul 20, 2004·The Journal of Biological Chemistry·Jason K YanoEric F Johnson
Sep 17, 2004·Biochemical and Biophysical Research Communications·Eriko UchidaKuniyo Inouye
Oct 7, 2004·Biochemical and Biophysical Research Communications·Raku ShinkyoKuniyo Inouye
Mar 5, 2008·Biochemistry·Hiroshi SugimotoToshiyuki Sakaki
May 31, 2008·Journal of Molecular Biology·Natallia StrushkevichHee-Won Park
Apr 4, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yoshiaki YasutakeTomohiro Tamura
Apr 9, 2009·Bioscience, Biotechnology, and Biochemistry·Tadashi FujiiMasashi Ito
May 20, 2009·Biochemical and Biophysical Research Communications·Yoshikazu FujiiTomohiro Tamura
Dec 8, 2009·Journal of Molecular Biology·Andrew J AnnaloraC David Stout

❮ Previous
Next ❯

Citations

Oct 12, 2013·Journal of Industrial Microbiology & Biotechnology·Jun-Gyu BanEung-Soo Kim
Jan 28, 2014·BoneKEy Reports·Roger Bouillon, Tatsuo Suda
May 23, 2014·Applied Microbiology and Biotechnology·Rita Bernhardt, Vlada B Urlacher
Oct 12, 2013·Chembiochem : a European Journal of Chemical Biology·Yoshiaki YasutakeTomohiro Tamura
May 17, 2013·Chembiochem : a European Journal of Chemical Biology·Wataru KitagawaTomohiro Tamura
Mar 15, 2015·Computational Biology and Chemistry·Swi See AngMohd Basyaruddin Abdul Rahman
Mar 7, 2018·The Protein Journal·Swi See AngMariam-Aisha Fatima
Dec 16, 2019·Applied Microbiology and Biotechnology·Panhong YuanLong Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.